B6.129S-Atoh1^tm6.1Hzo/J

Stock number: 013595
Product name: Atoh1^FLAG
Research areas: Cancer Research, Neurobiology Research, Research Tools

Description

These Atoh1^FLAG mice contain three C-terminal FLAG tags fused to the 3' end of the atonal homolog 1 gene.

Detailed description

These Atoh1^FLAG mice contain three c-terminal FLAG tags fused to the 3' end of the atonal homolog 1 (Atoh1) gene. Homozygous mice are viable and fertile. Atoh1^FLAG expression is evident evident in all Atoh1 expressing cells including cerebellar granule neuron precursors (GNPs) where it binds the active transcriptional enhancer region of the GLI-Kruppel family member GLI2 (Gli2) gene. These mice may be useful for studying postnatal cerebellar development and regulation of Sonic Hedgehog-induced medulloblastoma.

Development

A targeting vector was designed to fuse three c-terminal FLAG tags to the 3' end of the atonal homolog 1 (Atoh1) gene, followed by a frt-flanked neomycin (neo) selection cassette in reverse orientation to the gene. This construct was electroporated into 129S7/SvEvBrd-Hprt1^b-m2-derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym mutant mice to remove the neo cassette. The resulting Atoh1^FLAG mice were subsequently mated with inbred C57BL/6 mice for at least 8 generations. Upon arrival at The Jackson Laboratory, these mice were bred with C57BL/6J mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Atoh1^tm6.1Hzo
Name: targeted mutation 6.1, Huda Y Zoghbi
MGI accession ID: MGI:4420925
Generation method: Targeted
Attributes: Reporter; Inserted expressed sequence; No functional change
Marker symbol: Atoh1
Marker name: atonal bHLH transcription factor 1
Chromosome: 6
Strain of origin: 129S7/SvEvBrd-Hprt1^b-m2
Molecular note: A cDNA encoding for a version of the gene with three C-terminal FLAG tags and an FRT-NEO-FRT selection cassette was inserted, replacing the entire coding sequence. The selection cassette was then removed by flp mediated recombination. Expression of the tagged protein in the cerebellum but not the cortex was confirmed by chromatin immunoprecipitation analysis.