JAX Mouse Strain Datasheet - 013594

B6.129S-Atoh1^{tm5.1(Cre/PGR)Hzo}/J

Stock No:: 013594

Cryo Recovery

Overview

This knockin mutation replaces the coding sequence of the (Atoh1) gene with a modified Cre-recombinase-progesterone receptor fusion protein (Cre-PR). The fusion gene activity is inducible by administration of RU486.

Donating Investigator

Huda Zoghbi, Baylor College of Medicine

Genetic overview

Genetic Background	Generation

Atoh1^{tm5.1(cre/PGR)Hzo}

Allele Type	Gene Symbol	Gene Name
Targeted (Inducible, Recombinase-expressing)	Atoh1	atonal bHLH transcription factor 1

View Genetics

Research Applications

Developmental Biology Research
Neurobiology Research
Research Tools

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

A targeting vector was designed to replace the coding sequence of the atonal homolog 1 (Atoh1) gene with a modified Cre-recombinase-progesterone receptor fusion protein (Cre-PR), abolishing gene function. Homozygous Math1^Cre*PR mice die before birth due to central respiratory failure. Heterozygous mice are viable,fertile, and normal in size. The Math1^Cre*PR/+ allele has expression of the Cre*PR fusion protein under control of the Math1 promoter/enhancer elements. Cre*PR fusion gene activity is inducible; observed only 6-12 hours after administration of RU486, a competitive progesterone receptor antagonist. As such, when Math1^Cre*PR/+ mice are bred with mice containing loxP-flanked sequence, RU486-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Math1-expressing cells of the offspring. As such Math1 is expressed in the hindbrain, specifically in the conscious proprioceptive nuclei of the cortex at E10.5, and in most unconscious proprioceptive lineages in the cerebellum at E12.5-E14.5. These mutant mice are useful for the studying the characterization and time of formation of Math1-expressing cell lineages, as well as genetic and developmental links between proprioception, interoception, hearing, and arousal.

Development

A targeting vector was designed to insert a modified Cre-recombinase-progesterone receptor fusion protein (Cre-PR) downstream of the start codon of the atonal homolog 1 (Atoh1) gene, followed by a frt-flanked neomycin (neo) selection cassette. This construct was electroporated into 129S7/SvEvBrd-Hprt1^b-m2-derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym mutant mice to remove the neo cassette. The resulting Math1^Cre*PR/+ mice were subsequently mated with inbred C57BL/6 mice for at least 8 generations. Upon arrival at The Jackson Laboratory, these mice were bred with C57BL/6J mice (Stock No. 000664) for at least one generation to establish the colony.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	RU486-inducible Cre-mediated recombination will result in deletion of Math1 in the hindbrain, specifically in the conscious proprioceptive nuclei of the cortex at E10.5, and in most unconscious proprioceptive lineages in the cerebellum at E12.5-E14.5.

Control Suggestions

Wild-type from the colony

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Rose MF; Ahmad KA; Thaller C; Zoghbi HY. 2009. Excitatory neurons of the proprioceptive, interoceptive, and arousal hindbrain networks share a developmental requirement for Math1. Proc Natl Acad Sci U S A 106(52):22462-7. PubMed: 20080794 MGI: J:155932

View All References

Genetics

Atoh1^{tm5.1(cre/PGR)Hzo}

Allele Symbol: Atoh1^{tm5.1(cre/PGR)Hzo}

Allele Name	targeted mutation 5.1, Huda Y Zoghbi
Allele Type	Targeted (Inducible, Recombinase-expressing)
Allele Synonym(s)	Atoh1^tm5Hzo; Math1^cre*PR
Gene Symbol and Name	Atoh1, atonal bHLH transcription factor 1
Gene Synonym(s)	ATH1; HATH1; Hath1; MATH-1; Math1; RGD1565171; bHLHa14
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	RU486-inducible Cre-mediated recombination will result in deletion of Math1 in the hindbrain, specifically in the conscious proprioceptive nuclei of the cortex at E10.5, and in most unconscious proprioceptive lineages in the cerebellum at E12.5-E14.5.
Strain of Origin	129S7/SvEvBrd-Hprt
Chromosome	6
Molecular Note	All coding sequence was replaced by a modified cre/progesterone receptor fusion construct followed by an frt flanked PGK-neomycin selection cassette. The selection cassette was subsequently excised by crossing to mice carrying Gt(ROSA)26Sor^tm1(FLP1)Dym. In the resulting mice, the inducible cre is under the control of the endogenous gene promoter.
Mutations Made By	Huda Zoghbi, Baylor College of Medicine

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Perinatal Lethality
  - Homozygous
Neurobiology Research
- Cerebellar Defects
- Cortical Defects
- Cre-lox System
  - Cre Recombinase expression in neural tissue
- Neurodevelopmental Defects
Research Tools
- Cre-lox System
  - Cre Recombinase Expression: Inducible
- Developmental Biology Research
  - Cre-lox System

Genotype: cre related

Research Tools
- Cre-lox System
- Genetics Research
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Atoh1^{tm5.1(cre/PGR)Hzo}/Atoh1⁺
involves: 129S4/SvJaeSor * 129S7/SvEvBrd

no phenotypic analysis

no phenotypic analysis
- mice are used in expression studies

References

Rose MF; Ahmad KA; Thaller C; Zoghbi HY. 2009. Excitatory neurons of the proprioceptive, interoceptive, and arousal hindbrain networks share a developmental requirement for Math1. Proc Natl Acad Sci U S A 106(52):22462-7. PubMed: 20080794 MGI: J:155932

Additional - Atoh1^{tm5.1(cre/PGR)Hzo} related

Driver EC; Sillers L; Coate TM; Rose MF; Kelley MW. 2013. The Atoh1-lineage gives rise to hair cells and supporting cells within the mammalian cochlea. Dev Biol 376(1):86-98. PubMed: 23318633 MGI: J:195176
Kim TH; Li F; Ferreiro-Neira I; Ho LL; Luyten A; Nalapareddy K; Long H; Verzi M; Shivdasani RA. 2014. Broadly permissive intestinal chromatin underlies lateral inhibition and cell plasticity. Nature 506(7489):511-5. PubMed: 24413398 MGI: J:207494
Klisch TJ; Xi Y; Flora A; Wang L; Li W; Zoghbi HY. 2011. In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development. Proc Natl Acad Sci U S A 108(8):3288-93. PubMed: 21300888 MGI: J:169298
Rose MF; Ren J; Ahmad KA; Chao HT; Klisch TJ; Flora A; Greer JJ; Zoghbi HY. 2009. Math1 is essential for the development of hindbrain neurons critical for perinatal breathing. Neuron 64(3):341-54. PubMed: 19914183 MGI: J:155760

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Atoh1<tm5.1(Cre/PGR)Hzo>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Atoh1tm5.1(cre/PGR)Hzo

Allele Symbol: Atoh1tm5.1(cre/PGR)Hzo

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: cre related

Mammalian Phenotype Terms by Genotype

Genotype: Atoh1tm5.1(cre/PGR)Hzo/Atoh1+involves: 129S4/SvJaeSor * 129S7/SvEvBrd

no phenotypic analysis

References

Additional - Atoh1tm5.1(cre/PGR)Hzo related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Atoh1^{tm5.1(cre/PGR)Hzo}

Allele Symbol: Atoh1^{tm5.1(cre/PGR)Hzo}

Genotype: Atoh1^{tm5.1(cre/PGR)Hzo}/Atoh1⁺
involves: 129S4/SvJaeSor * 129S7/SvEvBrd

Additional - Atoh1^{tm5.1(cre/PGR)Hzo} related