JAX Mouse Strain Datasheet - 013579

B6;129S6-Fam3b^tm1Bkht/J

Stock No:: 013579

Targeted Mutation

Cryo Recovery

Overview

Male mice homozygous for this knockout are glucose intolerant, have decreased hepatic insulin clearance and reduced hepatic glucose production in response to hyperinsulinemic conditions.

Donating Investigator

Brant R. Burkhardt, University of South Florida

Genetic overview

Genetic Background	Generation

Fam3b^tm1Bkht

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Fam3b	family with sequence similarity 3, member B

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Research Applications

Diabetes and Obesity Research

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by RT-PCR and Western blot analysis of pancreatic islets and small intestines from homozygotes. Male mice that are homozygous for the targeted mutation and 4 to 6 months of age exhibit higher blood glucose levels in glucose tolerance tests when compared to wildtype controls. Although, insulin sensitivity is normal in mutant mice, insulin levels are elevated after glucose stimulation (intraperitoneal glucose injection) due to decreased hepatic insulin clearance. Glucose intolerance is not observed in mutant female mice. Isolated pancreatic islets from male homozygotes have impaired glucose-stimulated insulin secretion and calcium signaling. Male homozygotes have reduced hepatic glucose production (HGP) and increased insulin-mediated HGP suppression conditions as determined by hyperinsulinemic-euglycemic clamp assays. Furthermore, male homozygotes display reduced expression of the critical gluconeogenic enzymes of glucose-6-phosphatase (G6P) and phosphoenolpyruvate carboxykinase (PEPCK) following a 24 hour fast as compared to wildtype controls as determined by RT-PCR.

Development

A targeting vector containing a Neo selection cassette was used to disrupt the transcriptional start site (TSS), secretion signal peptide (SP), and exons 1 and 2. The construct was electroporated into 129S6/SvEvTac derived TL1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygotes were intercrossed to produce homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Suggestions

Approximate Controls

101043 B6129SF1/J

Additional Information

Considerations for Choosing Controls

Selected References

Robert-Cooperman CE; Carnegie JR; Wilson CG; Yang J; Cook JR; Wu J; Young RA; Wolf BA; Burkhardt BR. 2010. Targeted disruption of pancreatic-derived factor (PANDER, FAM3B) impairs pancreatic beta-cell function. Diabetes 59(9):2209-18. PubMed: 20566664 MGI: J:167823

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Genetics

Fam3b^tm1Bkht

Allele Symbol: Fam3b^tm1Bkht

Allele Name	targeted mutation 1, Brant Burkhardt
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	PANDER^-
Gene Symbol and Name	Fam3b, family with sequence similarity 3, member B
Gene Synonym(s)	2-21; 9030624C24Rik; 9030624C24Rik; C21orf11; C21orf76; D16Jhu19e; D16Jhu19e; DNA segment, Chr 16, Johns Hopkins University 19; DNA segment, Chr 16, Johns Hopkins University 19, expressed; ORF9; ORF9; PANDER; PRED44; Pander; RGD1311662; RIKEN cDNA 9030624C24 gene; open reading frame 9
Strain of Origin	129S6/SvEvTac
Chromosome	16
Molecular Note	Exons 1 and 2 were replaced with a neomycin selection cassette in reverse orientation. This removed the transcriptional and translational start sites and the first 54 amino acids including the secretion signal peptide and helix A. mRNA was not detected in pancreatic islets, small intestine, liver, spleen, kidney, and brain by RT-PCR. Western blot analysis of pancratic islets demonstrated the absence of full length protein.
Mutations Made By	Brant Burkhardt, University of South Florida

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
- Hyperglycemia
  - males
- Impaired Insulin Processing

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Fam3b^tm1Bkht/Fam3b^tm1Bkht
involves: 129S6/SvEvTac * C57BL/6

homeostasis/metabolism phenotype

abnormal glucose homeostasis
- reduced hepatic glucose production during a hyperinsulinemic-euglycemic clamp

abnormal insulin clearance
- impaired insulin clearance as indicated by a reduced C-peptide to insulin ratio

decreased insulin secretion
- decreased insulin secretion response by isolated islets exposed to increased glucose concentrations

homeostasis/metabolism phenotype
- normal 24 and 48 hour fasting levels of glucose, insulin and glucagon

impaired glucose tolerance
- glucose intolerance in 3 month old male mice
- male mice show higher blood glucose in glucose tolerance tests at 4 and 6 months of age

increased circulating insulin level
- elevated insulin after glucose injection

endocrine/exocrine gland phenotype

decreased insulin secretion
- decreased insulin secretion response by isolated islets exposed to increased glucose concentrations

endocrine/exocrine gland phenotype
- normal islet architecture

References

Robert-Cooperman CE; Carnegie JR; Wilson CG; Yang J; Cook JR; Wu J; Young RA; Wolf BA; Burkhardt BR. 2010. Targeted disruption of pancreatic-derived factor (PANDER, FAM3B) impairs pancreatic beta-cell function. Diabetes 59(9):2209-18. PubMed: 20566664 MGI: J:167823

Additional - Fam3b^tm1Bkht related

Moak SL; Dougan GC; MarElia CB; Danse WA; Fernandez AM; Kuehl MN; Athanason MG; Burkhardt BR. 2014. Enhanced glucose tolerance in pancreatic-derived factor (PANDER) knockout C57BL/6 mice. Dis Model Mech 7(11):1307-15. PubMed: 25217499 MGI: J:217205
Robert-Cooperman CE; Wilson CG; Burkhardt BR. 2011. PANDER KO mice on high-fat diet are glucose intolerant yet resistant to fasting hyperglycemia and hyperinsulinemia. FEBS Lett 585(9):1345-9. PubMed: 21486565 MGI: J:171972

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Fam3b<tm1Bkht>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

Questions about Terms of Use

Licensing Information

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Fam3btm1Bkht

Allele Symbol: Fam3btm1Bkht

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Fam3btm1Bkht/Fam3btm1Bkhtinvolves: 129S6/SvEvTac * C57BL/6

homeostasis/metabolism phenotype

endocrine/exocrine gland phenotype

References

Additional - Fam3btm1Bkht related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Fam3b^tm1Bkht

Allele Symbol: Fam3b^tm1Bkht

Genotype: Fam3b^tm1Bkht/Fam3b^tm1Bkht
involves: 129S6/SvEvTac * C57BL/6

Additional - Fam3b^tm1Bkht related