These transgenic mice express a mutated human PER2 (S662G) gene and exhibit a shortened circadian period and an advanced sleep phase.
Louis Ptacek, University of California, San Francisco
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
These transgenic mice express a S662G (serine -> glycine) amino acid mutation in the human PER2 (period homolog 2 (Drosophila)) gene associated with familial advanced sleep phase syndrome (FASPS). The phenotype of these mice is reported to faithfully recapitulate the condition found in humans. Animals from both founder lines 864 and 867 demonstrate a short circadian period (21.6 hours in hemizygotes and 20.7 hours in homozygotes versus 23.7 hours in wildtype controls) and an advanced sleep phase. This strain may be useful in studies of familial advanced sleep phase syndrome (FASPS) and PER2 regulation of circadian rhythms.
The full-length sequence of the human PER2 gene carried in BAC RP11-139K17 was modified to change serine residue 662 to aspartic acid (S662G, AGT->GGT). It is not known what genetic background the mutation was created on, but the strain was backcrossed more than 15 times to C57BL/6 by the donating laboratory (see SNP data below). This is founder line 864.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Expressed Gene | PER2, period circadian regulator 2, human |
---|---|
Site of Expression |
Allele Name | transgene insertion 864, Louis J Ptacek |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | hPer2 S662G |
Gene Symbol and Name | Tg(PER2*S662G)864Ljp, transgene insertion 864, Louis J Ptacek |
Gene Synonym(s) | |
Promoter | PER2, period circadian regulator 2, human |
Expressed Gene | PER2, period circadian regulator 2, human |
Strain of Origin | Not Specified |
Chromosome | UN |
Molecular Note | The full-length sequence of the human PER2 gene carried in BAC RP11-139K17 was modified to change serine residue 662 to aspartic acid (S662G, AGT->GGT). |
Mutations Made By | Louis Ptacek, University of California, San Francisco |
When maintained as a live colony, hemizygotes may be bred.
When using the B6.Cg-Tg(PER2*S662G)864Ljp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013546 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(PER2*S662G)864Ljp |
Frozen Mouse Embryo | B6.Cg-Tg(PER2*S662G)864Ljp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(PER2*S662G)864Ljp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(PER2*S662G)864Ljp/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(PER2*S662G)864Ljp/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.