These mice possess loxP sites on either side of exon 2 of the Dnm2 (dynamin 2) gene. This strain may be useful in further characterizing the role of this gene in endocytosis.
Pietro De Camilli, Yale University School of Medicine, HHMI
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Dnm2 | dynamin 2 |
These mice possess loxP sites on either side of exon 2 of the Dnm2 (dynamin 2) gene. Mice that are homozygous for this floxed allele are viable, fertile, and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this floxed strain is useful in eliminating expression of Dnm2 in a tissue-specific fashion (documented in cultured fibroblasts).
Germline deletion results in embryonic lethality. This strain may be useful in further characterizing the role of this gene in endocytosis.
Exon 2 of the targeted gene was flanked by a loxP site in intron 1 and a FRT-flanked PGK-Neo sequence followed by a loxP site in intron 2. The targeting vector was electroporated into C57BL/6J-129S1/Sv-p+Tyr+Kitl + hybrid embryonic stem cells. Male chimeric offspring were mated to a C57BL/6 background FLPe recombinase-expressing deleter strain to excise the PGK-Neo sequence, leaving exon 2 flanked by loxP sites. The donating investigator reported that this strain was backcrossed to C57BL/6 (see SNP note below) for 10 generations by the donating laboratory.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Allele Name | targeted mutation 1.1, Pietro De Camilli |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Dnm2LoxP |
Gene Symbol and Name | Dnm2, dynamin 2 |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S1/SvImJ)F1 |
Chromosome | 9 |
Molecular Note | A loxP site was inserted upstream of exon 2 and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 2. Flp mediated recombination removed the neo cassette leaving exon 2 floxed. |
Mutations Made By | Pietro De Camilli, Yale University School of Medicine, HHMI |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129S1(Cg)-Dnm2tm1.1Pdc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013542 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Dnm2<tm1.1Pdc> |
Frozen Mouse Embryo | B6.129S1(Cg)-Dnm2<tm1.1Pdc>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1(Cg)-Dnm2<tm1.1Pdc>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1(Cg)-Dnm2<tm1.1Pdc>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S1(Cg)-Dnm2<tm1.1Pdc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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