B6.129S1(Cg)-Dnm2^tm1.1Pdc/J

Stock number: 013542
Research areas: Neurobiology Research, Research Tools

Description

These mice possess loxP sites on either side of exon 2 of the Dnm2 (dynamin 2) gene. This strain may be useful in further characterizing the role of this gene in endocytosis.

Detailed description

These mice possess loxP sites on either side of exon 2 of the Dnm2 (dynamin 2) gene. Mice that are homozygous for this floxed allele are viable, fertile, and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this floxed strain is useful in eliminating expression of Dnm2 in a tissue-specific fashion (documented in cultured fibroblasts).

Germline deletion results in embryonic lethality. This strain may be useful in further characterizing the role of this gene in endocytosis.

Development

Exon 2 of the targeted gene was flanked by a loxP site in intron 1 and a FRT-flanked PGK-Neo sequence followed by a loxP site in intron 2. The targeting vector was electroporated into C57BL/6J-129S1/Sv-p⁺Tyr⁺Kitl ⁺ hybrid embryonic stem cells. Male chimeric offspring were mated to a C57BL/6 background FLPe recombinase-expressing deleter strain to excise the PGK-Neo sequence, leaving exon 2 flanked by loxP sites. The donating investigator reported that this strain was backcrossed to C57BL/6 (see SNP note below) for 10 generations by the donating laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Allele

Symbol: Dnm2^tm1.1Pdc
Name: targeted mutation 1.1, Pietro De Camilli
MGI accession ID: MGI:4457365
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Dnm2
Marker name: dynamin 2
Chromosome: 9
Strain of origin: (C57BL/6 x 129S1/SvImJ)F1
Molecular note: A loxP site was inserted upstream of exon 2 and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 2. Flp mediated recombination removed the neo cassette leaving exon 2 floxed.