In this strain, codon 103 in exon 3 of the endogenous mitogen-activated protein kinase 8 interacting protein 1 (Mapk8ip1 or Jip1) gene is mutated from a Threonine (ACT) to an Alanine (GCC). These mice may be useful for studying MAP kinase signaling pathways and activation of JNK in response to metabolic stress.
Roger J Davis, University of Massachusetts Medical Sch.
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Not Specified) | Mapk8ip1 | mitogen-activated protein kinase 8 interacting protein 1 |
In this strain, codon 103 in exon 3 of the endogenous mitogen-activated protein kinase 8 interacting protein 1 (Mapk8ip1 or Jip1) gene was mutated from a Threonine (ACT) to an Alanine (GCC). Homozygous mice are viable, fertile, and normal in size. Jip1 is expressed in neurons, the β cells of pancreatic islets, lung, and kidney.
The JIP group of scaffold proteins bind to c-Jun N-terminal kinase (JNK), mitogen-activated protein kinase (MAPK) kinase 7 (MKK7), and to members of the mixed lineage protein kinase (MLK) group, and is required for JNK activation. Phosphorylation of codon 103 is essential for JIP1 to activate JNK, which results in diet-induced obesity and insulin resistance. The loss of JIP1-mediated JNK activation in these JIP1TA mice results in increased insulin sensitivity and fat oxidation when fed a high fat diet (HFD). These mice also exhibit a decrease in hepatic glucose production, accumulation of hepatic lipids and triglycerides. Hyperglycemia, hyperinsulinemia, and food intake are reduced. No difference in weight gain, glucose intolerance, glucose-induced insulin release, insulin-stimulated whole-body glucose turnover, glycoloysis, or glycogen synthesis is observed when compared to wildtype mice on a HFD. These mice may be useful for studying MAP kinase signaling pathways and activation of JNK in response to metabolic stress.
A targeting construct was made containing exon 3 of the mitogen-activated protein kinase 8 interacting protein 1 (Mapk8ip1 or Jip1) gene. Codon 103 in exon 3 was mutated from a Threonine (ACT) to an Alanine (GCC), and a loxP-flanked neomycin (neo) resistance cassette was placed downstream of exon 3. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric mice were bred. These mice were then bred to mice carrying a Prm1-cre transgene (founder line PC3) on a C57BL/6J x 129/Sv background to remove the floxed selection cassette. These Jip1TA mice were backcrossed for at least 10 generations to C57BL/6J. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 3.1, Roger J Davis |
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Allele Type | Targeted (Not Specified) |
Allele Synonym(s) | Mapk8ip1T103A |
Gene Symbol and Name | Mapk8ip1, mitogen-activated protein kinase 8 interacting protein 1 |
Gene Synonym(s) | |
Promoter | Mapk8ip1, mitogen-activated protein kinase 8 interacting protein 1, mouse, laboratory |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | A targeting construct was made containing exon 3 of the mitogen-activated protein kinase 8 interacting protein 1 (Mapk8ip1 or Jip1) gene. Codon 103 in exon 3 was mutated from a Threonine (ACT) to an Alanine (GCC) (T103A), and a loxP-flanked neomycin (neo) resistance cassette was placed downstream of exon 3. Cre mediated recombination removed the neo cassette. |
Mutations Made By | Roger Davis, University of Massachusetts Medical Sch. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129-Mapk8ip1tm3.1Rjd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013536 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Mapk8ip1<tm3.1Rjd> |
Frozen Mouse Embryo | B6.129-Mapk8ip1<tm3.1Rjd>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Mapk8ip1<tm3.1Rjd>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Mapk8ip1<tm3.1Rjd>/J | $3373.50 |
Frozen Mouse Embryo | B6.129-Mapk8ip1<tm3.1Rjd>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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