CD4-Cre transgenic mice contain CD4 enhancer, promoter and silencer sequences driving the expression of a Cre recombinase gene. These mice may be useful for generating conditional mutations in CD4-expressing tissues.
Alexander Chervonsky, The University of Chicago
Christopher B. Wilson, University of Washington
Genetic Background | Generation |
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001976 NOD/ShiLtJ |
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Allele Type |
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Transgenic (Recombinase-expressing) |
CD4-Cre transgenic mice contain CD4 enhancer, promoter and silencer sequences driving the expression of a Cre recombinase gene. Hemizygotes are viable and fertile. Specifically, Cre recombinase expression is observed in CD4-expressing T cells during sequential stages of T cell development in lymphoid tissues. Cre expression commences at the very late double-negative stage and generally results in >99% deletion of loxP flanked genes by the double-positive stage of T-cell development. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These mice may be useful for studying CD4 T cell maturation and proliferation.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The CD4-Cre transgene was designed with the CD4 enhancer, promoter and silencer sequence driving the expression of a Cre recombinase gene. This transgene was injected into oocytes and mice from founder line 1 were bred to C57BL/6 mice. This strain had been maintained on a C57BL/6 background when it was obtained by Dr. Alexander Chervonsky at The University of Chicago. These mice were then backcrossed with NOD/ShiLt inbred mice for at least 11 generations. Upon arrival at the Jackson Laboratory Repository, these mice were bred to NOD/ShiLt inbred mice (Stock No. 001976) for at least one generation.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | T cells |
Allele Name | transgene insertion 1, Christopher B Wilson |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | CD4CRE; CD4cre; CD4-Cre; CD4-Cre+; CD4-creTg; CD4p-Cre; Tg(CD4-cre)1Cw1 |
Gene Symbol and Name | Tg(Cd4-cre)1Cwi, transgene insertion 1, Christopher B Wilson |
Gene Synonym(s) | |
Promoter | Cd4, CD4 antigen, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | T cells |
Strain of Origin | (C57BL/6 x DBA/2)F2 |
Chromosome | UN |
Molecular Note | Cre recombinase is expressed under the control of mouse Cd4 regulatory elements. The Cd4 enhancer, promoter and silencer collectively drive expression at sequential stages specifically of CD4+ T cell development. |
Mutations Made By | Christopher Wilson, University of Washington |
When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) mice from the colony or to NOD/ShiLt inbred mice (Stock No. 001976).
When using the NOD.CD4-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #013234 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non Carrier for Tg(Cd4-cre)1Cwi |
Frozen Mouse Embryo | NOD.Cg-Tg(Cd4-cre)1Cwi/2AchJ | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Tg(Cd4-cre)1Cwi/2AchJ | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Tg(Cd4-cre)1Cwi/2AchJ | $3373.50 |
Frozen Mouse Embryo | NOD.Cg-Tg(Cd4-cre)1Cwi/2AchJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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