These Abl1flox/flox mutant mice posses loxP sites flanking exons 5-6 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1. This strain may be useful for studying the growth, development, and physiology of cardiac tissue.
Stephen P. Goff, Columbia University
These Abl1flox/flox mutant mice posses loxP sites flanking exons 5-6 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1. Mice that are homozygous for this allele are viable, fertile, and normal in size. When these Abl1flox/flox mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 5-6 deleted in the cre-expressing tissue. This strain may be useful for studying the growth, development, and physiology of cardiac tissue.
A targeting vector was designed to insert a loxP site upstream of exon 5 and a loxP site downstream of exon 6 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1. The construct also contained a floxed-neomycin (neo) resistance cassette in exon 6. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre-recombinase expression plasmid to delete the neo cassette. Resulting ES cells contained multiple gene rearrangments; intact floxed-exons 5-6, intact floxed-neo cassette, or excision of exons 5-6 and the neo cassette. Correctly targeted ES cells, containing floxed-exons 5-6, were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6J females. The resulting Abl1flox/flox mice were then backcrossed to C57BL/6J for at least eight generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 2.1, Stephen P Goff|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Abl1, c-abl oncogene 1, non-receptor tyrosine kinase|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A loxP site was inserted at the end of intron 4 and a neo cassette flanked by two loxP sites were inserted into the middle of intron 6. The neo cassette was deleted by transiently transfection of an expression vector of Cre recombinase in the ES cells. Two independent heterozygous ES cell clones.|
|Mutations Made By|| |
Stephen Goff, Columbia University
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129P2-Abl1tm2.1Goff/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013224 in your Materials and Methods section.
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