B6.129P2-Abl1^tm2.1Goff/J

Stock number: 013224
Research areas: Cardiovascular Research, Internal/Organ Research, Research Tools

Description

These Abl1^flox/flox mutant mice posses loxP sites flanking exons 5-6 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1. This strain may be useful for studying the growth, development, and physiology of cardiac tissue.

Detailed description

These Abl1^flox/flox mutant mice posses loxP sites flanking exons 5-6 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1. Mice that are homozygous for this allele are viable, fertile, and normal in size. When these Abl1^flox/flox mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 5-6 deleted in the cre-expressing tissue. This strain may be useful for studying the growth, development, and physiology of cardiac tissue.

Development

A targeting vector was designed to insert a loxP site upstream of exon 5 and a loxP site downstream of exon 6 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1. The construct also contained a floxed-neomycin (neo) resistance cassette in exon 6. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre-recombinase expression plasmid to delete the neo cassette. Resulting ES cells contained multiple gene rearrangments; intact floxed-exons 5-6, intact floxed-neo cassette, or excision of exons 5-6 and the neo cassette. Correctly targeted ES cells, containing floxed-exons 5-6, were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6J females. The resulting Abl1^flox/flox mice were then backcrossed to C57BL/6J for at least eight generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Abl1^tm2.1Goff
Name: targeted mutation 2.1, Stephen P Goff
MGI accession ID: MGI:4850040
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Abl1
Marker name: c-abl oncogene 1, non-receptor tyrosine kinase
Chromosome: 2
Strain of origin: 129P2/OlaHsd
Molecular note: A loxP site was inserted at the end of intron 4 and a neo cassette flanked by two loxP sites were inserted into the middle of intron 6. The neo cassette was deleted by transiently transfection of an expression vector of Cre recombinase in the ES cells. Two independent heterozygous ES cell clones.