This visual arrestin knock out mouse strain may be useful in studies of photoreceptor adaptation, phototransduction, and retinopathy.
Jeannie Chen, University of Southern California
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Sag | S-antigen, retina and pineal gland (arrestin) |
Breeding colonies at The Jackson Laboratory are maintained in normal light conditions. We will attempt to maintain this colony in shelf positions that allow the least amount of light possible (i.e., lower shelves), but can not guarantee the extent of retinal damage (if any) in the mice we provide. For experimental use, we strongly suggest investigators use the mice we provide for breeding purposes rather than experiments. This allows each investigator to generate their own experimental offspring in light conditions ideal for their own respective experiments.
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mutant mice are photosensitive and if not maintained under low light conditions will develop progressive retinal degeneration. No gene product (protein) is detected by Northern blot analysis of retinas from homozygous mice maintained in cyclic (12 hour light: 12 hour dark) conditions. Photoreceptor loss in homozygotes maintained in cyclic light conditions begins at approximately 100 days of age, progressing to loss of more than half of the photoreceptors by 1 year of age. After 1 week of constant light exposure, homozygotes lose 30% of photoreceptors; after 3 weeks of constant light exposure, more than 60% of photoreceptors are lost. Homozygotes maintained under cyclic light conditions exhibit disorganized and 25% shorter retinal rod outer segment layer, as well as reduced levels of retinal rhodopsin. Recovery phase response to light flash stimulation of the retina is significantly longer in homozygotes, although light adaptation function is normal.
IMPORTANT NOTE: Breeding colonies at The Jackson Laboratory are maintained in normal light conditions. We will attempt to maintain this colony in shelf positions that allow the least amount of light possible (i.e., lower shelves), but can not guarantee the extent of retinal damage (if any) in the mice we provide. For experimental use, we strongly suggest investigators use the mice we provide for breeding purposes rather than experiments. This allows each investigator to generate their own experimental offspring in light conditions ideal for their own respective experiments.
A targeting vector containing MC1neopA cassette was used to disrupt part of the 5' promoter and exons 1 and 2. The construct was electroporated into 129/SvEv derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The donating investigator reports these mice were then backcrossed to C57BL/6 for five generations (see SNP results below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
In March 2011, a 32 SNP (single nucleotide polymorphism) panel analysis with markers covering all 19 chromosomes and the X chromosome was performed on the first generation of rederived mice at The Jackson Laboratory Repository. This revealed markers that were not C57BL/6 allele-type at nine loci (representing seven different chromosomes: 1, 3, 6, 8, 12, 13, and 17). The marker on chromosome 17 (~34 Mbp) is neither C57BL/6 nor 129S allele-type. The other eight markers may be 129S (or other) allele-type. These data suggest the mice sent to The Jackson Laboratory Repository were not fully backcrossed onto the C57BL/6 genetic background, and are on a more mixed genetic background of C57BL/6 and 129S with contributions from either A, C3H/He, SJL, SWR, and/or other unknown lines. Furthermore, this data might suggest the mice are on a mixed C57BL/6;129S genetic background with some other contamination on chromosome 17 (that may or may not affect the MHC genes).
Allele Name | targeted mutation 1, Jeannie Chen |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | arr1-; Arrb1tm1Jnc; arrestin- |
Gene Symbol and Name | Sag, S-antigen, retina and pineal gland (arrestin) |
Gene Synonym(s) | |
Strain of Origin | 129S/SvEv |
Chromosome | 1 |
Molecular Note | Part of the 5' promoter and exons 1 and 2 were replaced with a neomycin resistance cassette via homologous recombination. Gene expression was undetectable in rod cells of homozygous mutant animals as shown by Western blot analysis. |
Mutations Made By | Jeannie Chen, University of Southern California |
When maintaining a live colony, these mice can be bred as homozygotes.
IMPORTANT NOTE: Breeding colonies at The Jackson Laboratory are maintained in normal light conditions. We will attempt to maintain this colony in shelf positions that allow the least amount of light possible (i.e., lower shelves), but can not guarantee the extent of retinal damage (if any) in the mice we provide. For experimental use, we strongly suggest investigators use the mice we provide for breeding purposes rather than experiments. This allows each investigator to generate their own experimental offspring in light conditions ideal for their own respective experiments.
When using the STOCK Sagtm1Jnc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013197 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Sag<tm1Jnc> |
Frozen Mouse Embryo | STOCK Sag<tm1Jnc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Sag<tm1Jnc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Sag<tm1Jnc>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Sag<tm1Jnc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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