Overview

This visual arrestin knock out mouse strain may be useful in studies of photoreceptor adaptation, phototransduction, and retinopathy.

Donating Investigator

Jeannie Chen, University of Southern California

Genetic overview

Genetic Background	Generation

Sag^tm1Jnc

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Sag	S-antigen, retina and pineal gland (arrestin)

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Research Applications

Research Tools
Sensorineural Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Important Note

Breeding colonies at The Jackson Laboratory are maintained in normal light conditions. We will attempt to maintain this colony in shelf positions that allow the least amount of light possible (i.e., lower shelves), but can not guarantee the extent of retinal damage (if any) in the mice we provide. For experimental use, we strongly suggest investigators use the mice we provide for breeding purposes rather than experiments. This allows each investigator to generate their own experimental offspring in light conditions ideal for their own respective experiments.

Detailed Description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mutant mice are photosensitive and if not maintained under low light conditions will develop progressive retinal degeneration. No gene product (protein) is detected by Northern blot analysis of retinas from homozygous mice maintained in cyclic (12 hour light: 12 hour dark) conditions. Photoreceptor loss in homozygotes maintained in cyclic light conditions begins at approximately 100 days of age, progressing to loss of more than half of the photoreceptors by 1 year of age. After 1 week of constant light exposure, homozygotes lose 30% of photoreceptors; after 3 weeks of constant light exposure, more than 60% of photoreceptors are lost. Homozygotes maintained under cyclic light conditions exhibit disorganized and 25% shorter retinal rod outer segment layer, as well as reduced levels of retinal rhodopsin. Recovery phase response to light flash stimulation of the retina is significantly longer in homozygotes, although light adaptation function is normal.

IMPORTANT NOTE: Breeding colonies at The Jackson Laboratory are maintained in normal light conditions. We will attempt to maintain this colony in shelf positions that allow the least amount of light possible (i.e., lower shelves), but can not guarantee the extent of retinal damage (if any) in the mice we provide. For experimental use, we strongly suggest investigators use the mice we provide for breeding purposes rather than experiments. This allows each investigator to generate their own experimental offspring in light conditions ideal for their own respective experiments.

Development

A targeting vector containing MC1neopA cassette was used to disrupt part of the 5' promoter and exons 1 and 2. The construct was electroporated into 129/SvEv derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The donating investigator reports these mice were then backcrossed to C57BL/6 for five generations (see SNP results below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

In March 2011, a 32 SNP (single nucleotide polymorphism) panel analysis with markers covering all 19 chromosomes and the X chromosome was performed on the first generation of rederived mice at The Jackson Laboratory Repository. This revealed markers that were not C57BL/6 allele-type at nine loci (representing seven different chromosomes: 1, 3, 6, 8, 12, 13, and 17). The marker on chromosome 17 (~34 Mbp) is neither C57BL/6 nor 129S allele-type. The other eight markers may be 129S (or other) allele-type. These data suggest the mice sent to The Jackson Laboratory Repository were not fully backcrossed onto the C57BL/6 genetic background, and are on a more mixed genetic background of C57BL/6 and 129S with contributions from either A, C3H/He, SJL, SWR, and/or other unknown lines. Furthermore, this data might suggest the mice are on a mixed C57BL/6;129S genetic background with some other contamination on chromosome 17 (that may or may not affect the MHC genes).

Control Suggestions

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Xu J; Dodd RL; Makino CL; Simon MI; Baylor DA; Chen J. 1997. Prolonged photoresponses in transgenic mouse rods lacking arrestin. Nature 389(6650):505-9PubMed: 9333241 MGI: J:43294

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Genetics

Sag^tm1Jnc

Allele Symbol: Sag^tm1Jnc

Allele Name	targeted mutation 1, Jeannie Chen
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	arr1-; Arrb1^tm1Jnc; arrestin^-
Gene Symbol and Name	Sag, S-antigen, retina and pineal gland (arrestin)
Gene Synonym(s)
Strain of Origin	129S/SvEv
Chromosome	1
Molecular Note	Part of the 5' promoter and exons 1 and 2 were replaced with a neomycin resistance cassette via homologous recombination. Gene expression was undetectable in rod cells of homozygous mutant animals as shown by Western blot analysis.
Mutations Made By	Jeannie Chen, University of Southern California

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Sensorineural Research
  - retinal degeneration
Sensorineural Research
- Retinal Degeneration
- Eye Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Sag^tm1Jnc/Sag^tm1Jnc
involves: 129S/SvEv

mammalian phenotype

vision/eye phenotype
- Normal - mice exhibit normal cone electrophysiology

nervous system phenotype

abnormal retinal rod cell outer segment morphology
- Normal - however, raising mice in darkness reduces the effect
- the rod outer segment is disorganized and 25% shorter than in wild-type mice

vision/eye phenotype

abnormal retinal rod cell outer segment morphology
- Normal - however, raising mice in darkness reduces the effect
- the rod outer segment is disorganized and 25% shorter than in wild-type mice

abnormal rod electrophysiology
- Normal - however, response to light adaptation is normal
- prolonged decay of slow-phase response is associated with increased meta-II decay
- slow phase of the dim-flash response is 10-fold longer than in the Arrb1^tm1Jnc Rho^tm1Shda double homozygotes
- rods exhibit an abnormal falling phase of the flash response compared to in wild-type mice
- recovery from flash response is prolonged in the final phase unlike wild type mice

References

Xu J; Dodd RL; Makino CL; Simon MI; Baylor DA; Chen J. 1997. Prolonged photoresponses in transgenic mouse rods lacking arrestin. Nature 389(6650):505-9PubMed: 9333241 MGI: J:43294

Additional - Sag^tm1Jnc related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Sag
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
IMPORTANT NOTE: Breeding colonies at The Jackson Laboratory are maintained in normal light conditions. We will attempt to maintain this colony in shelf positions that allow the least amount of light possible (i.e., lower shelves), but can not guarantee the extent of retinal damage (if any) in the mice we provide. For experimental use, we strongly suggest investigators use the mice we provide for breeding purposes rather than experiments. This allows each investigator to generate their own experimental offspring in light conditions ideal for their own respective experiments.
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Sag^tm1Jnc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013197 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Sag<tm1Jnc>

Related Products and Services

Frozen Mouse Embryo	STOCK Sag<tm1Jnc>/J	$2595.00
Frozen Mouse Embryo	STOCK Sag<tm1Jnc>/J	$2595.00
Frozen Mouse Embryo	STOCK Sag<tm1Jnc>/J	$3373.50
Frozen Mouse Embryo	STOCK Sag<tm1Jnc>/J	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Sagtm1Jnc

Allele Symbol: Sagtm1Jnc

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Sagtm1Jnc/Sagtm1Jncinvolves: 129S/SvEv

mammalian phenotype

nervous system phenotype

vision/eye phenotype

References

Additional - Sagtm1Jnc related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Sag^tm1Jnc

Allele Symbol: Sag^tm1Jnc

Genotype: Sag^tm1Jnc/Sag^tm1Jnc
involves: 129S/SvEv

Additional - Sag^tm1Jnc related