B6.129-Aip^tm2Bra/J

Stock number: 013195
Research areas: Research Tools

Description

These Aip^fx/fx conditional (floxed) mutant mice possess loxP sites flanking exons 3 through 6, and may be useful in generating conditional mutations for studying early embryonic development, vascular development, and toxicology.

Detailed description

These mice possess loxP sites on either side of exons 3 through 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 through 6 deleted in the cre-expressing tissue(s).

Development

A FRT-site flanked targeting vector containing a neo cassette and an upstream loxP site was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 6 of the targeted gene, and another loxP site was inserted upstream of exon 3. The construct was electroporated into 129/SvJ derived GS1 embryonic stem (ES) Correctly targeted ES cells were injected into recipient blastocysts. The mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym/J (Stock No. 003946) to remove the FRT-site flanked neo cassette. Mice that retained the loxP site flanked exons 3 to 6 were then bred to C57BL/6J mice to remove the Gt(ROSA)26Sor^tm1(FLP1)Dym allele. The mice were backcrossed to C57BL/6J for 10 generations and them maintained by homozygous intercross for 4 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Aip^tm2Bra
Name: targeted mutation 2, Christopher A Bradfield
MGI accession ID: MGI:4867827
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Hypomorph
Marker symbol: Aip
Marker name: aryl-hydrocarbon receptor-interacting protein
Chromosome: 19
Strain of origin: 129/Sv
Molecular note: A loxP site was inserted upstream of exon 3 and an FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 6. Reduced protein expression was confirmed by western blot analysis on heart, liver, kidney, spleen, and thymus extracts. This allele is hypomorphic.