In this strain, the allele replaces alternate exon 1 and common exon 2 of the regulatory associated protein of mTOR (Mechanistic target of rapamycin), complex 1 (Rptor) gene with an internal ribosome entry site (IRES)-lacZ (β-galactosidase), and a neomycin resistance (neo) cassette, abolishing gene function. These mice may be useful for studying raptor-mTOR complex signaling associated with cell growth, cell proliferation/differentiation, cell survival, cancer, diabetes, aging, tuberous sclerosis complex (TSC), lymphangioleiomyomatosis (LAM), Cowden disease, Peutz-Jeghers syndrome (PJS), neurofibromatosis, and familial cardiac hypertrophy.
David Sabatini, Whitehead Institute
In this strain, the allele replaces alternate exon 1 and common exon 2 of the regulatory associated protein of mTOR (Mechanistic target of rapamycin), complex 1 (Rptor) gene with an internal ribosome entry site (IRES)-lacZ(β-galactosidase), and a neomycin resistance (neo) cassette, abolishing gene function. Heterozygous mice are viable, fertile, and normal in size, while homozygous mice die by e6.5. Raptor forms the complex mTORC1 with mLST8 (mTOR associated Protein, LST8 homolog) and mTOR, which is critical for the growth of the inner cell mass and trophoblast cell outgrowth during early embryonic development. When Raptor-/- blastocysts are implanted, 25% can implant but all fail to expand and differentiate. In culture, by day 4 the cells of the blastocysts stop growing and by day 7 the cells are detached and dying. β-gal staining is evident in embryonic cells expressing Raptor. These mice may be useful for studying raptor-mTOR complex signaling associated with cell growth, cell proliferation/differentiation, cell survival, cancer, diabetes, aging, tuberous sclerosis complex (TSC), lymphangioleiomyomatosis (LAM), Cowden disease, Peutz-Jeghers syndrome (PJS), neurofibromatosis, and familial cardiac hypertrophy.
A targeting vector was designed to replace alternate exon 1 and common exon 2 of the regulatory associated protein of MTOR (Mechanistic target of rapamycin), complex 1 (Rptor) gene with an internal ribosome entry site (IRES)-lacZ (β-galactosidase) followed by a neomycin resistance (neo) cassette. The construct was electroporated into 129S5/SvEv-derived embryonic stem (ES) cells. The donating investigator reports that correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were backcrossed at least 8 times to C57BL/6 mice (see SNP note below) to generate a colony of Rptor+/- mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Lexicon Genetics|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Rptortm1Lex; targeted mutation 1, Lexicon Genetics|
|Gene Symbol and Name||Rptor, regulatory associated protein of MTOR, complex 1|
|Gene Synonym(s)||Rap; raptor; RGD1311784; RIKEN cDNA 4932417H02 gene; Mip1; KOG1; mKIAA1303; 4932417H02Rik; 4932417H02Rik|
|Site of Expression||lacZ is expressed in embryonic cells expressing Raptor.|
|Strain of Origin||129S/SvEvBrd|
|Molecular Note||Alternate exon 1 and common exon 2 were replaced with an IRES-lacZ/neo cassette.|
When maintaining a live colony, heterozygous mice may be bred to wildtype littermates from the colony or C57BL/6J inbred mice (Stock No. 000664). Homozygous embryos die by e6.5.
When using the B6.129S5-Rptortm1Lex/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013191 in your Materials and Methods section.
|Heterozygous oir wildtype for Rptor<tm1Lex>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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