Overview

This secretory trap mutation abolishes endogenous mTOR (Mechanistic target of rapamycin) gene function and expresses a neomycin (neo) resistance cassette/simian virus 40 (SV40) polyadenylylation signal fusion protein. These mice may be useful for studying mTOR complex signaling associated with cell growth, cell proliferation/differentiation, cell survival, cancer, diabetes, aging, tuberous sclerosis complex (TSC), lymphangioleiomyomatosis (LAM), Cowden disease, Peutz-Jeghers syndrome (PJS), neurofibromatosis, and familial cardiac hypertrophy.

Donating Investigator

David Sabatini, Whitehead Institute

Genetic overview

Genetic Background	Generation

Mtor^{Gt(OST92090)Lex}

Allele Type	Gene Symbol	Gene Name
Gene trapped	Mtor	mechanistic target of rapamycin kinase

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Research Applications

Developmental Biology Research
Research Tools
Cancer Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

This secretory trap mutation abolishes endogenous mTOR (Mechanistic target of rapamycin) gene function and expresses a neomycin (neo) resistance cassette/simian virus 40 (SV40) polyadenylylation signal fusion protein. Heterozygous mice are viable, fertile, and normal in size, while homozygous mice die by e6.5. mTOR forms the complex mTORC1 with mLST8 (mTOR associated protein (LST8 homolog)) and Rptor (regulatory associated protein of mTOR, complex 1), which is critical for the growth of the inner cell mass and trophoblast cell outgrowth during early embryonic development. mTOR also forms the complex mTORC2 with mLST8 and Rictor (Rptor independent companion of mTOR, complex 2), which is critical for midgestational embryonic development. The inner cell mass and trophoblast giant cells fail to expand in explanted mTOR-/- blastocysts. By day 4 the cells of the blastocysts stop growing and by day 7 the cells are detached and dying. These mice may be useful for studying mTOR complex signaling associated with cell growth, cell proliferation/differentiation, cell survival, cancer, diabetes, aging, tuberous sclerosis complex (TSC), lymphangioleiomyomatosis (LAM), Cowden disease, Peutz-Jeghers syndrome (PJS), neurofibromatosis, and familial cardiac hypertrophy.

Development

A "secretory trap" targeting vector was used replace the endogenous Mechanistic target of rapamycin (mTOR) targeted gene with a neomycin (neo) resistance cassette followed by the simian virus 40 polyadenylylation signal. This gene trap targeting vector integrated into intron 1 at base position 488 of the mTOR locus via electroporation into 129S5/SvEvBrd-derived Lex-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were backcrossed at least 8 times to C57BL/6 mice to generate a colony of mTOR^+/- mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Control Suggestions

Wild-type from the colony

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Guertin DA; Stevens DM; Thoreen CC; Burds AA; Kalaany NY; Moffat J; Brown M; Fitzgerald KJ; Sabatini DM. 2006. Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1. Dev Cell 11(6):859-71PubMed: 17141160 MGI: J:137592

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Genetics

Mtor^{Gt(OST92090)Lex}

Allele Symbol: Mtor^{Gt(OST92090)Lex}

Allele Name	gene trap OST92090, Lexicon Genetics
Allele Type	Gene trapped
Allele Synonym(s)	gene trap OST92090, Lexicon Genetics; Mtor^{Gt(OST92090)Lex}
Gene Symbol and Name	Mtor, mechanistic target of rapamycin kinase
Gene Synonym(s)	FKBP-rapamycin-associated protein FRAP; AI327068; flat; RAFT1; RAPT1; 2610315D21Rik; flat; Frap1; FRAP; FRAP1; FRAP2; RIKEN cDNA 2610315D21 gene; expressed sequence AI327068; FK506 binding protein 12-rapamycin associated protein 1; flat-top; 2610315D21Rik; Frap1; SKS; mechanistic target of rapamycin (serine/threonine kinase)
Strain of Origin	129S5/SvEvBrd
Chromosome	4
Molecular Note	The gene trapping vector containing a neo cassette was inserted into intron 1 at position 488.
Mutations Made By	David Sabatini, Whitehead Institute

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Lymphoid Tissue Defects
- Embryonic Lethality (Homozygous)
- Internal/Organ Defects
Research Tools
- Diabetes and Obesity Research
Cancer Research
- Increased Tumor Incidence

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Mtor^{Gt(OST92090)Lex}/Mtor^{Gt(OST92090)Lex}
involves: 129S5/SvEvBrd

cellular phenotype

absent inner cell mass proliferation
- inner cell mass explants fail to expand in culture unlike wild-type explants

embryo phenotype

abnormal trophoblast giant cell morphology
- explants fail to expand in culture unlike wild-type explants

absent inner cell mass proliferation
- inner cell mass explants fail to expand in culture unlike wild-type explants

References

Guertin DA; Stevens DM; Thoreen CC; Burds AA; Kalaany NY; Moffat J; Brown M; Fitzgerald KJ; Sabatini DM. 2006. Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1. Dev Cell 11(6):859-71PubMed: 17141160 MGI: J:137592

Additional - Mtor^{Gt(OST92090)Lex} related

Technical Support

Contact Technical Support

Genotyping Protocols
- High Resolution Melting: Mtor^{Gt(OST92090)Lex}
- Standard PCR: Mtor^{Gt(OST92090)Lex}
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or C57BL/6J inbred mice (Stock No. 000664). Homozygous embryos die by e6.5.
- Additional Breeding and Husbandry Support
Citation
When using the B6.129S5-Mtor^{Gt(OST92090)Lex}/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013190 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Mtor<Gt(OST92090)Lex>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Email: TechTran@jax.org

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

MtorGt(OST92090)Lex

Allele Symbol: MtorGt(OST92090)Lex

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: MtorGt(OST92090)Lex/MtorGt(OST92090)Lexinvolves: 129S5/SvEvBrd

cellular phenotype

embryo phenotype

References

Additional - MtorGt(OST92090)Lex related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Mtor^{Gt(OST92090)Lex}

Allele Symbol: Mtor^{Gt(OST92090)Lex}

Genotype: Mtor^{Gt(OST92090)Lex}/Mtor^{Gt(OST92090)Lex}
involves: 129S5/SvEvBrd

Additional - Mtor^{Gt(OST92090)Lex} related