These mice possess loxP sites on either side of exons 6 and 7 of the Ahi1 (Abelson helper integration site 1) targeted gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of the gene. This strain may be useful in studies of retinopathy, nephronophthisis and Joubert syndrome.
Joseph G Gleeson, University of California, San Diego
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Ahi1 | Abelson helper integration site 1 |
These mice possess loxP sites on either side of exons 6 and 7 of the Ahi1 (Abelson helper integration site 1) targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of the gene. This strain may be useful in studies of retinopathy, nephronophthisis and Joubert syndrome.
A loxP-flanked PGK-Neomycin resistance cassette was introduced to intron 7 and exons 6 and 7 of the targeted gene were flanked by loxP sites in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. Crosses with an EIIa-Cre strain were used to excise the PGK-neomycin cassette leaving floxed exons 6 and 7. This strain was maintained on a mixed 129, C57BL/6 and other unknown background by the donating laboratory.
Allele Name | targeted mutation 2.1, Joseph G Gleeson |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Ahi1, Abelson helper integration site 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 10 |
Molecular Note | A loxP site was inserted upstream of exon 6 and a floxed neo cassette was inserted downstream of exon 7. Cre-mediated recombination removed the neo cassette leaving exon 6 through 7 floxed. |
Mutations Made By | Joseph Gleeson, University of California, San Diego |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the STOCK Ahi1tm2.1Jgg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013167 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ahi1<tm2.1Jgg> |
Frozen Mouse Embryo | STOCK Ahi1<tm2.1Jgg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Ahi1<tm2.1Jgg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Ahi1<tm2.1Jgg>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Ahi1<tm2.1Jgg>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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