STOCK Ahi1^tm2.1Jgg/J

Stock number: 013167
Research areas: Research Tools

Description

These mice possess loxP sites on either side of exons 6 and 7 of the Ahi1 (Abelson helper integration site 1) targeted gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of the gene. This strain may be useful in studies of retinopathy, nephronophthisis and Joubert syndrome.

Detailed description

These mice possess loxP sites on either side of exons 6 and 7 of the Ahi1 (Abelson helper integration site 1) targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of the gene. This strain may be useful in studies of retinopathy, nephronophthisis and Joubert syndrome.

Development

A loxP-flanked PGK-Neomycin resistance cassette was introduced to intron 7 and exons 6 and 7 of the targeted gene were flanked by loxP sites in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Crosses with an EIIa-Cre strain were used to excise the PGK-neomycin cassette leaving floxed exons 6 and 7. This strain was maintained on a mixed 129, C57BL/6 and other unknown background by the donating laboratory.

Allele

Symbol: Ahi1^tm2.1Jgg
Name: targeted mutation 2.1, Joseph G Gleeson
MGI accession ID: MGI:4437788
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ahi1
Marker name: Abelson helper integration site 1
Chromosome: 10
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A loxP site was inserted upstream of exon 6 and a floxed neo cassette was inserted downstream of exon 7. Cre-mediated recombination removed the neo cassette leaving exon 6 through 7 floxed.