In this strain the catalytic domains of the fucosyltransferase 4 (Fut4) gene and the fucosyltransferase 7 (Fut7) gene were replaced with neomycin resistance (neo) cassettes, abolishing gene function. These mice may be useful for studying leukocyte recruitment and lymphocyte homing.
Dr. John Lowe, U of Michigan Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Fut7 | fucosyltransferase 7 |
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Fut4 | fucosyltransferase 4 |
In this strain the catalytic domains of the fucosyltransferase 4 (Fut4) gene and the fucosyltransferase 7 (Fut7) gene were replaced with neomycin resistance (neo) cassettes, abolishing gene function. Mice homozygous for both alleles are viable, fertile, and normal in size. These mice lack inflammation-dependent leukocyte recruitment; specifically neutrophils, eosinophils, monocytes, T cells and dendritic cells are unable to migrate to extravascular sites of acute and chronic inflammation. The peripheral nodes in these mice do not support normal B lymphocyte or naive T lymphocyte homing. FucTIV-null defects in leukocyte E- and P-selectin counterreceptor activity and HEV-born L-selectin ligand activity are reversed in the double null mutant mice. Neutrophils in the doubly null mice, unlike FucT-7VII null leukocytes, are virtually devoid of E- and P-selectin ligand activities. These mice also exhibit extreme leukocytosis characterized by decreased neutrophil turnover and increased neutrophil production. These mice may be useful for studying leukocyte recruitment and lymphocyte homing.
A targeting vector was designed to replace the catalytic domain of the fucosyltransferase 4 (Fut4) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to (C57BL/6J x DBA/2J)F1 females. These mice were intercrossed and subsequently backcrossed to C57BL/6J for at least 12 generations to create a colony of FucTIV(-/-) mice.
A targeting vector was designed to replace the catalytic domain of the fucosyltransferase 7 (Fut7) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to (C57BL/6J x DBA/2J)F1 females. These mice were intercrossed and subsequently backcrossed to C57BL/6J for at least 12 generations to create a colony of FucTVII(-/-) mice.
FucTIV and FucTVII mice were bred together to generate a mouse strain that is homozygous for both alleles (FucTIV(-/-)/FucTVII(-/-)). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, John Lowe |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | FucT-VII (-) |
Gene Symbol and Name | Fut7, fucosyltransferase 7 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 2 |
Molecular Note | Sequence encoding the catalytic domain was disrupted by the insertion of a PGK-neo cassette. |
Allele Name | targeted mutation 1, John Lowe |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | FucT-IV- |
Gene Symbol and Name | Fut4, fucosyltransferase 4 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 9 |
Molecular Note | 436 bp of genomic sequence encoding the catalytic domain was replaced with a neomycin selection cassette. |
When maintaining a live colony, double homozygous mice may be bred together, double heterozygous mice may be bred to wildtype mice from the colony, or to C57BL/6J inbred mice (Stock No. 000664).
When using the B6.129S2(Cg)-Fut7tm1Jbl Fut4tm1Jbl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013152 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Fut7<tm1Jbl>, Heterozygous for Fut4<tm1Jbl> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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