Mouse Strain Datasheet - 013141
Mouse Strain Datasheet - 013141
The D2.B10 (DBA/2-congenic) Dmdmdx mouse (also referred to as DBA/2J-mdx or D2-mdx mice) may be a superior Duchenne muscular dystrophy model as it better recapitulates several of the human characteristics of DMD myopathology (lower hind limb muscle weight, fewer myofibers, increased fibrosis and fat accumulation, and muscle weakness) relative to strains with this mutant allele on other genetic backgrounds.
IMR Colony, The Jackson Laboratory
| Genetic Background | Generation |
|---|---|
|
?+F13
|
| Allele Type | Gene Symbol | Gene Name |
|---|---|---|
| Spontaneous | Dmd | dystrophin, muscular dystrophy |
Duchenne muscular dystrophy (DMD) is a progressive muscular disorder caused by an imbalance between muscle degeneration and regeneration resulting in muscle degeneration, necrosis, accumulation of fat and fibrosis, and insufficient regeneration/loss of myofibers. The genetic cause of DMD are mutations of the dystrophin muscular dystrophy gene (DMD) on the X chromosome. The Dmdmdx mutation in mice has a termination codon in exon 23 that is predicted to result in a truncated protein. Heterozygous females are viable and fertile with no gross phenotypic abnormalities. Homozygous females and hemizygous males are viable and fertile with myopathic features of DMD; although the myopathology is both less severe than the human disease course and variable by mouse strain genetic background.
The muscle pathology observed for C57BL/10ScSn-Dmdmdx mice (also referred to as C57BL/10-mdx ; Stock No. 001801) includes active fiber necrosis, cellular infiltration, a wide range of fiber sizes, and numerous centrally nucleated regenerating fibers. However, adult C57BL/10-mdx mice fail to exhibit several of the skeletal muscle characteristics of DMD (such as smaller number of myofibers, accumulation of fat and fibrosis, insufficient myofiber regeneration, and loss of muscle weight). In addition, the C57BL/10 inbred strain is shown to possess greater muscle satellite cell self-renewal ability/efficiency than that of the DBA/2 inbred strain which may increase muscle regeneration/attenuate muscular dystrophy phenotype for MD mutations maintained on the C57BL/10 genetic background. As such, The Jackson Laboratory Repository created this DBA/2J-congenic Dmdmdx mouse model, D2.B10-Dmdmdx/J (also referred to as DBA/2J-mdx or D2-mdx; Stock No. 013141).
These DBA/2J-mdx mice may be expected to have a phenotype similar to other Dmdmdx mouse models. That is, when compared to C57BL/10-mdx animals, DBA/2-congenic Dmdmdx mice exhibit additional DMD characteristics such as lower hind limb muscle weight, fewer myofibers, increased fibrosis and fat accumulation, and remarkable muscle weakness. The DBA/2-congenic Dmdmdx phenotype is attributed to diminished myofiber regeneration (rather than accelerated myofiber degeneration). Unlike C57BL/10-mdx mice, no increased incidence of spontaneous rhabdomyosarcoma-like tumors are reported for the DBA/2-congenic Dmdmdx model.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for these DBA/2J-mdx mice (Stock No. 013141). It should be noted that the phenotype could vary from that originally described for C57BL/10-mdx (Stock No. 001801). We will modify the strain description if necessary as published results become available.
The spontaneous mutation "X chromosome-linked muscular dystrophy" (mdx) has as a C-to-T transition (resulting in a termination codon) at position 3185 within exon 23 of the dystrophin muscular dystrophy gene (Dmd) on the X chromosome. C57BL/10ScSn-Dmdmdx (C57BL/10-mdx) mice are available from The Jackson Laboratory as Stock No. 001801. In 2010, some C57BL/10-mdx mice were backcrossed to DBA/2J inbred mice (Stock No. 000671) for several generations using a marker-assisted, speed congenic approach to generate the D2.B10-congenic strain (also referred to as DBA/2J-mdx) as Stock No. 013141.
| Allele Name | X linked muscular dystrophy |
|---|---|
| Allele Type | Spontaneous |
| Allele Synonym(s) | Dmdmdx; X linked muscular dystrophy |
| Gene Symbol and Name | Dmd, dystrophin, muscular dystrophy |
| Gene Synonym(s) | Duchenne muscular dystrophy; CMD3B; DXS164; DXS230; DXS239; DXS268; DXS272; X-linked muscular dystrophy; BMD; pke; pke; DXS142; DXS206; DXS270; mdx; DXS269; MRX85; dys; X-linked muscular dystrophy; pyruvate kinase expression; Dp427; Dp71; mdx |
| Strain of Origin | C57BL/10ScSn |
| Chromosome | X |
| Molecular Note | This mutation arose in 1981 in a C57BL/10ScSn colony at University of Leicester. A C-to-T transition occurred at position 3185, resulting in a termination codon in place of a glutamine codon. This mutation is predicted to produce a truncated protein. |
The Dmdmdx mutant allele is on the X chromosome. Mutant mice were bred to DBA/2J inbred mice (Stock No. 000671) for many generations using a marker-assisted, speed congenic approach to establish this congenic strain. When maintaining the live congenic colony, heterozygous or homozygous females may be bred with hemizygous males.
When using the D2.B10-Dmdmdx/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013141 in your Materials and Methods section.
| Service | Genotype | Price |
|---|---|---|
| X linked - Females Heterozygous and males wildtype for Dmd<mdx>, 1 pair minimum |
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
| Frozen Mouse Embryo | $2,595.00 per straw or vial |
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