C.129S6(Cg)-Npsr1^tm1Bhk/J

Stock number: 013133
Research areas: Cardiovascular Research, Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

In this strain, exon 4 of the endogenous neuropeptide S receptor 1 (Npsr1 or GPRA) gene is replaced with a neo cassette, abolishing ligand binding function. These GPRA mutant mice may be useful for studying the induction of asthma-like disease, smooth muscle constriction, and airway function.

Detailed description

In this strain, exon 4 of the endogenous neuropeptide S receptor 1 (Npsr1 or GPRA) gene is replaced with a neo cassette, abolishing ligand binding function. Homozygous (GPRA^-/-) mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. GPRA^-/- mice on a 129/SvEv genetic background show an attenuated response when challenged with the cholinergic receptor-dependent bronchoconstricting agent thromboxane. These GPRA mutant mice may be useful for studying the induction of asthma-like disease, smooth muscle constriction, and airway function.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector was designed to replace exons 4 encoding the neuropeptide S receptor 1 (Npsr1 or G protein-coupled receptor for asthma susceptibility,Gpra) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to B6D2 females and subsequently to BALB/C mice to generate a colony of Gpra^-/- mice. These mice were subsequently backcrossed at least 12 generations to BALB/c background. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ (Stock No. 000651) for at least one generation to establish the colony.

Allele

Symbol: Npsr1^tm1Bhk
Name: targeted mutation 1, Beverly H Koller
MGI accession ID: MGI:3832372
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Npsr1
Marker name: neuropeptide S receptor 1
Chromosome: 9
Strain of origin: 129S6/SvEvTac
Molecular note: Exon 4 was replaced with a neo cassette. Northern blot analysis confirmed the expression of a truncated transcript.