B6129S-Del(7Slx1b-Sept1)4Aam/J

Stock number: 013128
Product name: 16p11.2df
Research areas: Developmental Biology Research, Mouse/Human Gene Homologs, Neurobiology Research

Description

These mutant mice possess an engineered deletion spanning approximately 0.39 Mb on mouse Chromosome 7, a region that shares conserved synteny with the Autism spectrum disorders critical interval on human Chromosome 16. This mutant mouse may be useful in studying Autism and other associated disorders.

Detailed description

These mutant mice possess an engineered deletion spanning approximately 0.39 Mb on mouse Chromosome 7. The region involved encompasses a chromosomal segment, between the GIY-YIG domain containing 2 (Giyd2) gene and the septin 1(Sept1) loci, that shares conserved synteny with the Autism spectrum disorders critical interval on human Chromosome 16 (the 16p11.2 region). Mice homozygous for the deletion are embryonic lethal. Mice carrying one copy of the deletion prove to be viable, but do exhibit some postnatal lethality. It can be expected that approximately half of df/+ mice will be lost between birth and weaning. These mice exhibit neuroanatomical and behavioral phenotypes reminiscent of human autism. This mutant mouse may be useful in studying Autism and other associated disorders.

(Mice bearing the reciprocal duplication are also available (see Stock No. 013129))

Development

Chromosome-engineering cassettes were inserted into mouse Chromosome 7 of 129S7/SvEvBrd-Hprt1^b-m2-derived AB2.2 embryonic stem (ES) cells, bracketing a span of approximately 0.39 Mb between the GIY-YIG domain containing 2 (Giyd2) gene and the septin 1(Sept1) loci. The cassette placed at the proximal locus contained Giyd2, a puromycin resistance gene, a loxP site, a 5' portion of an hprt minigene and an agouti transgene. The cassette placed at the distal locus contained the Sept1 gene, a tyrosinase minigene, a 3' portion of an hprt minigene, a loxP site and neomycin resistance gene. Double-targeted ES cells were subjected to transient cre recombinase expression with subsequent selection of recombinants by using hypoxanthine aminopterin thymidine (HAT) media. Correctly targeted ES cells, containing one copy of Chromosome 7 with the targeted sequence deleted (df) and one copy with the targeted sequence duplicated (dp), were injected into C57BL/6J blastocysts and the resulting chimeric mice were mated to C57BL/6J mice. The resulting offspring were genotyped and maintained as two separate lines of df and dp mice. The df mice were mated to C57BL/6J mice for at least two generations. Males were then sent to The Jackson Laboratory Repository. Sperm from these males were frozen. Individual aliquots of the frozen sperm were used to establish df mice on a mixed B6129S genetic background (Stock No. 013128). A frozen sperm aliquot was used to fertilize B6129SF1/J oocytes (Stock No. 101043). The resulting df mice were subsequently bred to B6129SF1/J hybrid mice each generation.

Allele

Symbol: Del(7Slx1b-Sept1)4Aam
Name: deletion, Chr 7, Alea A Mills 4
MGI accession ID: MGI:4462822
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Del(7Slx1b-Sept1)4Aam
Marker name: deletion, Chr 7, Alea A Mills 4
Chromosome: 7
Strain of origin: 129S7/SvEvBrd-Hprt^b-m2
Molecular note: Chromosome-engineering cassettes were inserted into mouse Chromosome 7, bracketing a span of approximately 0.39 Mb between the SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (Smg1) and the aldolase A, fructose-bisphosphate (Aldoa) loci. The cassette placed at the proximal locus contained Smg1, a puromycin resistance gene, a loxP site, a 3' portion of an hprt minigene and an agouti transgene. The cassette placed at the distal locus contained the Aldoa gene, a tyrosinase minigene, a 5' portion of an hprt minigene, a loxP site and neomycin resistance gene. Double-targeted ES cells were subjected to transient cre recombinase expression with subsequent selection of recombinants by using hypoxanthine aminopterin thymidine (HAT) media to delete the targeted region.