B6.129-Asic3^tm1Wsh/J

Stock number: 013127
Research areas: Neurobiology Research, Sensorineural Research

Description

Homozygous Asic3 knockout mice display alterations in normal and pain-related cutaneous mechanosensitivity and inflammatory pain sensation, visual loss associated with night blindness, and age-induced hearing loss. This strain may be useful in further elucidating the role of this gene in mechanosensation.

Detailed description

Homozygous Asic3 (amiloride-sensitive cation channel 3) targeted mutation mice display alterations in normal and pain-associated cutaneous mechanosensitivity and inflammatory pain sensation, visual loss associated with night blindness, and age-induced hearing loss. Mice show increased sensitivity of mechanoreceptors detecting light touch. Sensitivity of mechanoreceptors responding to noxious pinch is decreased as is the response of acid- and noxious heat-sensitive nociceptors. No mRNA is detectable by RT-PCR in the dorsal root ganglion. Protein is not found in trigeminal and dorsal root ganglion. This strain may be useful in further elucidating the role of this gene in mechanosensation.

Development

A region of the targeted gene containing the first exon (which includes the start ATG codon), the first transmembrane segment and part of the extracellular domain, was replaced with a loxP-flanked neomycin resistance cassette in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 for more than 10 generations by the donating laboratory.

Allele

Symbol: Asic3^tm1Wsh
Name: targeted mutation 1, Michael J Welsh
MGI accession ID: MGI:2429689
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Asic3
Marker name: acid-sensing (proton-gated) ion channel 3
Chromosome: 5
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exon 1 was replaced with a floxed neomycin selection cassette inserted by homologous recombination. The deleted region contained the translational start codon and encoded the transmembrane domain and a portion of the extracellular domain. Transcript was undetected in homozygous mutant mice by RT-PCR analysis.