Overview

Also Known As:RAG-1^-; UBI-GFP

Rag1-deficient ubiquitin-GFP mice on the C57BL/6 genetic background (B6.Rag1;UBC-GFP) combine lymphocyte-deficiency with widespread green fluorescent protein expression. These mice allow in vivo fluorescent tracking of non-lymphocyte cell populations. They are also suitable for use as recipient hosts for implanted embryos/tissues/cells from non-fluorescent mice.

In 2020, the UBC-GFP transgene was determined to have integrated at Chr17:29,435,589 - a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H2-K1) - and is closely linked to H-2^b MHC haplotype. See Important Note for additional details.

Donating Investigator

IMR Colony, The Jackson Laboratory

Genetic overview

Genetic Background	Generation

Rag1^tm1Mom

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Rag1	recombination activating gene 1

Tg(UBC-GFP)30Scha

Allele Type
Transgenic (Reporter)

View Genetics

Research Applications

Research Tools
Internal/Organ Research
Cancer Research
Hematological Research
Immunology, Inflammation and Autoimmunity Research
Virology Research
Neurobiology Research
Diabetes and Obesity Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Important Note

Important Note regarding transgene insertion and linkage to H-2^b MHC haplotype: In Liu et al. 2020 J Immunol. 204:1982 [PMID:32122998], it was discovered that the UBC-GFP transgene integrated into chromosome 17 at nucleotide position 29,435,589 (reference sequence NC_000083.6; GRCm38.p6) - this is a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H2-K1). The number of transgene copies was not determined. Furthermore, this determined that BALB/cBy-congenic UBC-GFP transgenic mice (N11+ from Stock No. 007076) retained the H-2^b MHC haplotype from the genetic background upon which it was created (C57BL/6), rather than the H-2^d MHC haplotype of BALB/cBy.
Although close to the H-2 locus, segregation is possible. For experiments where MHC haplotype is a factor, researchers should determine if the UBC-GFP transgenic mice are H-2^b or H-2^d.

Detailed Description

In 2020, the UBC-GFP transgene was determined to have integrated at Chr17:29,435,589 - a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H2-K1) - and is closely linked to H-2^b MHC haplotype. See Important Note for additional details.

Rag1-deficient ubiquitin-GFP mice on the C57BL/6 genetic background (B6.Rag1^-/-;UBC-GFP) combine lymphocyte-deficiency with widespread green fluorescent protein expression.

Mice homozygous for both the Rag1 null allele and the UBC-GFP transgene (Rag1^-/-;UBC-GFP^+/+) are viable and fertile. Double homozygous mice produce no mature T cells or B cells, and have widespread GFP expression that is uniform within cell lineage. GFP expression varies between different cell types; hematopoietic cells have high expression levels compared with other cells. Red blood cells from UBC-GFP homozygotes should fluoresce at approximately twice the level compared to UBC-GFP hemizygotes.

Similar to other immunodeficient strains, maintaining Rag1-deficient mice in high health status (specific pathogen-free) vivaria promotes overall colony health. If Rag1-deficient animals are maintained in low health barrier rooms, the use of medicated water (sulfatrim/trimethoprim-sulfa) is suggested to increase overall colony health.

C57BL/6J-congenic mice harboring this Rag1 null allele are described and available from The Jackson Laboratory Repository as Stock No. 002216.

UBC-GFP transgenic mice from founder line 30 on a C57BL/6 genetic background are described and available from The Jackson Laboratory Repository as Stock No. 004353. The transgene insertion on chromosome 17 results in linkage to H-2^b MHC haplotype (see Important Note).

Development

These B6.Rag1;UBC-GFP mice harbor the Rag1 null allele (Rag1^tm1Mom) and a UBC-GFP transgene (Tg(UBC-GFP)30Scha).
To generate the B6.Rag1;UBC-GFP mutant line (Stock No. 013115), C57BL/6J-congenic Rag1 null homozygous mice (Stock No. 002216) were bred with C57BL/6.UBC-GFP homozygous mice (Stock No. 004353) for three generations and selecting offspring for homozygosity. The Jackson Laboratory Repository will maintain the live Rag1.UBC-GFP colony by breeding mice homozygous for the Rag1 null allele and homozygous for the UBC-GFP transgene.

The Rag1 null allele (Rag1^tm1Mom) was designed by Dr. Peter Mombaerts (in the laboratory of Dr. Susumu Tonegawa at the Center for Cancer Research, Massachusetts Institute of Technology) with a PGK-neo cassette replacing 1356 bp (encoding the nuclear localization signal and the zinc-finger motif) at the 5' end of the recombination activating gene 1 coding sequence on chromosome 2. C57BL/6J-congenic mice harboring this Rag1 null allele are described and available from The Jackson Laboratory Repository as Stock No. 002216.

The ubiquitin-GFP transgene (UBI-GFP or UBC-GFP) was designed by Dr. Brian C. Schaefer (while at National Jewish Medical and Research Center) with an enhanced green fluorescent protein open reading frame under the control of the human ubiqutin C promoter. Transgenic mice from founder line 30 on a C57BL/6 genetic background are described and available from The Jackson Laboratory Repository as Stock No. 004353.
In 2020, the UBC-GFP transgene was determined to have integrated at Chr17:29,435,589 - a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H2-K1) - and is closely linked to H-2^b MHC haplotype. See Important Note for additional details.

In 2018, a 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Three of the 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating, including the rd8 recessive mutation associated with retinal degeneration on Chromosome 1 within the Crb1 locus. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6N;C57BL/6J genetic background.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	Certain hematopoietic cell types display distinct expression levels of GFP. Expression is uniform within a cell type lineage and remains constant throughout development.

Control Suggestions

Approximate Controls

Depending upon the experiment, the following may also be appropriate control strain(s):
--B6.129S7-Rag1^tm1Mom/J (Stock No. 002216)
--C57BL/6-Tg(UBC-GFP)30Scha/J (Stock No. 004353)
--C57BL/6J inbred mice (Stock No. 000664)
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Liu S; Lockhart JR; Fontenard S; Berlett M; Ryan TM. 2020. Mapping the Chromosomal Insertion Site of the GFP Transgene of UBC-GFP Mice to the MHC Locus. J Immunol 204(7):1982-1987PubMed: 32122998 MGI: J:287278

View All References

Genetics

Rag1^tm1Mom

Allele Symbol: Rag1^tm1Mom

Allele Name	targeted mutation 1, Peter Mombaerts
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Rag-; Rag^1tm1Mom; RAG-1-; Rag1^-; RAG1^null; Rag-1KO
Gene Symbol and Name	Rag1, recombination activating gene 1
Gene Synonym(s)
Site of Expression	expression is seen in bone marrow derived cell lines.
Strain of Origin	129S7/SvEvBrd-Hprt⁺
Chromosome	2
Molecular Note	A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.
Mutations Made By	Peter Mombaerts, Max Planck Research Unit for Neurogenetics

Tg(UBC-GFP)30Scha

Allele Symbol: Tg(UBC-GFP)30Scha

Allele Name	transgene insertion 30, Brian Schaefer
Allele Type	Transgenic (Reporter)
Allele Synonym(s)	Tg(UBCGFP)30Scha; UBC-GFP; Ub-GFP; UBI-EGFP; UBI-GFP; uGFP
Gene Symbol and Name	Tg(UBC-GFP)30Scha, transgene insertion 30, Brian Schaefer
Gene Synonym(s)
Promoter	UBC, ubiquitin C, human
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	Certain hematopoietic cell types display distinct expression levels of GFP. Expression is uniform within a cell type lineage and remains constant throughout development.
Strain of Origin	C57BL/6
Chromosome	17
General Note	Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express GFP in all tissues examined. Expression levels vary between certain hematapoietic cell types. GFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher GFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous transgenic mice fluoresce at approximately twice the level of cells from hemizygous mice.
Molecular Note	The transgene contains an enhanced green fluorescent protein open reading frame under the control of the human ubiquitin C promoter. The transgene integrated into chromosome 17 at nucleotide position 29,435,589 (reference sequence NC_000083.6; GRCm38.p6) - this is a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H-2 K1).
Mutations Made By	Brian Schaefer, Uniformed Services Univ. Health Sciences

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Rag1^tm1Mom related

Research Tools
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
- Toxicology Research
  - xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Cancer Research
- Toxicology
  - B and T cell deficiency, xenograft/transplant host
Hematological Research
- Immunological Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
- T Cell Receptor Signaling Defects
  - B and T cell deficiency
- Inflammation
  - B and T cell deficiency

Cancer Research
- Toxicology
  - B and T cell deficiency, xenograft/transplant host
  - xenograft/transplant host
Research Tools
- Genetics Research
  - Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
  - Tissue/Cell Markers: multiple
  - Tissue/Cell Markers: cell marker for bone marrow transplantation
  - Tissue/Cell Markers: spermatogonial transplantation marker
- Reproductive Biology Research
  - transplantation marker for embryonic and adult tissue
  - spermatogonial transplantation marker
- Toxicology Research
  - xenograft/transplant host
  - B and T cell deficiency, xenograft transplant host
- Fluorescent Proteins
- Immunology, Inflammation and Autoimmunity Research
  - T cell deficiency
  - T Cell Receptor Deficiency
  - T cell deficiency, xenograft/transplant host
  - production of B cells, antibodies T cell lines, and hybridomas
  - production of T cell lines and hybridomas
  - specific T cell deficiency
  - B and T cell deficiency
  - production of B cells and antibodies
  - B cell deficiency
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
  - T cell deficiency
  - production of B and T cells, antibodies, and hybridomas
  - production of T cells and hybridoma
  - specific T cell deficiency
  - B cell deficiency
  - production of B cells and antibodies
  - xenograft/transplant host
- Neurobiology Research
  - cell marker
- Developmental Biology Research
  - transplantation marker for embryonic and adult tissue
Virology Research
- B and T Cell Deficiency
  - AIDS research tool
Immunology, Inflammation and Autoimmunity Research
- T Cell Receptor Signaling Defects
  - B and T cell deficiency
  - B and T cell deficiency, xenograft/transplant host
- Lymphoid Tissue Defects
  - B and T cell deficiency
- Autoimmunity
  - B cell deficiency
  - B and T cell deficiency
- Immunodeficiency
  - B cell defects
  - B cell deficiency
  - B and T cell deficiency
  - T cell deficiency
  - specific T cell deficiency
- Inflammation
  - B and T cell deficiency
Internal/Organ Research
- Adrenal Cortex Defects
  - T cell deficiency
- Lymphoid Tissue Defects
  - B and T cell deficiency
  - T cell deficiency
- Thymus Defects
  - B and T cell deficient
Neurobiology Research
- Fluorescent protein expression in neural tissue
Diabetes and Obesity Research
- Islet Transplantation Studies
Hematological Research
- Immunological Defects
  - B and T cell deficiency

Mammalian Phenotype Terms by Genotype

References

Liu S; Lockhart JR; Fontenard S; Berlett M; Ryan TM. 2020. Mapping the Chromosomal Insertion Site of the GFP Transgene of UBC-GFP Mice to the MHC Locus. J Immunol 204(7):1982-1987PubMed: 32122998 MGI: J:287278

Additional - Rag1^tm1Mom related

Additional - Tg(UBC-GFP)30Scha related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Rag1Alternate1
- Genotyping resources and troubleshooting
Breeding Considerations

The Jackson Laboratory Repository will maintain the live Rag1.UBC-GFP colony by breeding together mice homozygous for both the Rag1 null allele and the UBC-GFP transgene.

Mice homozygous for the Rag1 null allele are lymphocyte-deficient. As such, and similar to other immunodeficient strains, maintenance in high health status (specific pathogen-free) vivaria promotes overall colony health. If Rag1-deficient animals are maintained in low health barrier rooms, the use of medicated water (sulfatrim/trimethoprim-sulfa) is suggested to increase overall colony health.
- Additional Breeding and Husbandry Support
Citation
When using the RAG-1^-; UBI-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #013115 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Homozygous for Rag1<tm1Mom>, Homozygote for Tg(UBC-GFP)30Scha,

Related Products and Services

Frozen Mouse Embryo	B6.Cg-Rag1<tm1Mom> Tg(UBC-GFP)30Scha/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6.Cg-Rag1<tm1Mom> Tg(UBC-GFP)30Scha/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6.Cg-Rag1<tm1Mom> Tg(UBC-GFP)30Scha/J Frozen Embryos	$3373.50
Frozen Mouse Embryo	B6.Cg-Rag1<tm1Mom> Tg(UBC-GFP)30Scha/J Frozen Embryos	$3373.50

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:RAG-1-; UBI-GFP

Donating Investigator

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Rag1tm1Mom

Allele Symbol: Rag1tm1Mom

Tg(UBC-GFP)30Scha

Allele Symbol: Tg(UBC-GFP)30Scha

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Rag1tm1Mom related

Mammalian Phenotype Terms by Genotype

References

Additional - Rag1tm1Mom related

Additional - Tg(UBC-GFP)30Scha related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Also Known As:RAG-1^-; UBI-GFP

Rag1^tm1Mom

Allele Symbol: Rag1^tm1Mom

Genotype: Rag1^tm1Mom related

Additional - Rag1^tm1Mom related