These mice possess loxP sites on either side of an exon encoding portions of both the PDZ1 and PDZ2 domains of the Dlg1 (discs, large homolog 1 (Drosophila); SAP97) gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful for further characterizaton of the targeted gene's synaptic function.
Richard L Huganir, Johns Hopkins University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Dlg1 | discs large MAGUK scaffold protein 1 |
These mice possess loxP sites on either side of an exon encoding portions of both the PDZ1 and PDZ2 domains of the Dlg1 (discs, large homolog 1 (Drosophila); SAP97) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.
Widespread deletion of expression leads to neonatal lethality due to failure of maxillar bone fusion. If deletion is specific to motor neurons (Stock No. 006600), neurite extension is suppressed. This strain may be useful for further characterizaton of the targeted gene's synaptic function.
When bred to mice carrying Tg(Cd4-cre)1Cwi (Stock No. 017336), Cre recombinase expression in the CD4-expressing T cells results un decreased Th1-associated cytokine production and increased Th2-associated cytokine production following anti-CD3 stimulation.
When bred to mice carrying Tg(Mpz-cre)26Mes (Stock No. 017927), Cre recombinase expression in Schwann cells results hypermyelination.
An FRT-flanked neomycin resistance cassette and a loxP site were introduced to the intron following the second exon of the the PDZ2 domain. A second loxP site was placed in an upstream intron, between two exons encoding the PDZ1 domain. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. The neomycin resistance cassette was excised through a cross with an beta actin (ACTB) promoter FLP strain on a C57BL/6 background, leaving a single floxed exon encoding portions of both the PDZ1 and PDZ2 domains. This strain was backcrossed once to C57BL/6 by the donating laboratory.
Allele Name | targeted mutation 1, Richard L Huganir |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | dlg1flox; SAP97flox |
Gene Symbol and Name | Dlg1, discs large MAGUK scaffold protein 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 16 |
Molecular Note | A loxP site was inserted upstream of the exon encoding the carboxy terminus of the first PDZ domain and the amino terminus of the second PZD domain and an frt flanked neo cassette with a 3' loxP site was inserted downstream of this exon. Germ line, flp mediated recombination was used to remove the neo cassette leaving the exon floxed. |
Mutations Made By | Richard Huganir, Johns Hopkins University School of Medicine |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129-Dlg1tm1Rlh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013097 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Dlg1<tm1Rlh> |
Frozen Mouse Embryo | B6;129-Dlg1<tm1Rlh>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Dlg1<tm1Rlh>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Dlg1<tm1Rlh>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Dlg1<tm1Rlh>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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