B6;129-Dlg1^tm1Rlh/J

Stock number: 013097
Research areas: Neurobiology Research, Research Tools

Description

These mice possess loxP sites on either side of an exon encoding portions of both the PDZ1 and PDZ2 domains of the Dlg1 (discs, large homolog 1 (Drosophila); SAP97) gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful for further characterizaton of the targeted gene's synaptic function.

Detailed description

These mice possess loxP sites on either side of an exon encoding portions of both the PDZ1 and PDZ2 domains of the Dlg1 (discs, large homolog 1 (Drosophila); SAP97) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

Widespread deletion of expression leads to neonatal lethality due to failure of maxillar bone fusion. If deletion is specific to motor neurons (Stock No. 006600), neurite extension is suppressed. This strain may be useful for further characterizaton of the targeted gene's synaptic function.

When bred to mice carrying Tg(Cd4-cre)1Cwi (Stock No. 017336), Cre recombinase expression in the CD4-expressing T cells results un decreased Th1-associated cytokine production and increased Th2-associated cytokine production following anti-CD3 stimulation.

When bred to mice carrying Tg(Mpz-cre)26Mes (Stock No. 017927), Cre recombinase expression in Schwann cells results hypermyelination.

Development

An FRT-flanked neomycin resistance cassette and a loxP site were introduced to the intron following the second exon of the the PDZ2 domain. A second loxP site was placed in an upstream intron, between two exons encoding the PDZ1 domain. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. The neomycin resistance cassette was excised through a cross with an beta actin (ACTB) promoter FLP strain on a C57BL/6 background, leaving a single floxed exon encoding portions of both the PDZ1 and PDZ2 domains. This strain was backcrossed once to C57BL/6 by the donating laboratory.

Allele

Symbol: Dlg1^tm1Rlh
Name: targeted mutation 1, Richard L Huganir
MGI accession ID: MGI:3817231
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Dlg1
Marker name: discs large MAGUK scaffold protein 1
Chromosome: 16
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A loxP site was inserted upstream of the exon encoding the carboxy terminus of the first PDZ domain and the amino terminus of the second PZD domain and an frt flanked neo cassette with a 3' loxP site was inserted downstream of this exon. Germ line, flp mediated recombination was used to remove the neo cassette leaving the exon floxed.