These clock fluorescent reporter (mPeriod2-DsRED) mutant mice express DsRed predominantly in the suprachiasmatic nuclei (SCN) with moderate expression in non-neuronal regions of the cerebral cortex, striatum, cerebellum and dentate gyrus. This strain may be useful for in vivo imaging of the SCN to monitor circadian clock timing.
Karl Obrietan, Ohio State University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice that are hemizygous for the clock fluorescent reporter transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of mPeriod2-DsRED is predominantly directed to the suprachiasmatic nuclei (SCN) with moderate expression in non-neuronal regions of the cerebral cortex, striatum, cerebellum and dentate gyrus. There is limited or no expression in the CA1 area of the hippocampus, Purkinje cell layer of the cerebellum or the piriform cortex and in neurons from other brain regions.
This mutant mouse strain may be useful for in vivo imaging of the SCN to monitor circadian clock timing.
A bacterial articificial chromosone (BAC) #RP24-391L2 containing the mouse Per2 gene was modified by the introduction of a DsRED.T3-NLS-PEST cassette. The DsRED.T3 variant carries 8 point mutations relative to the parent DsRED protein and exhibits rapid maturation kinetics. To further improve the kinetics of DsRED, the PEST domain of the mouse ornithine carboxylase gene was added to the last codon of DsRED and a nuclear localization sequence (NLS) was cloned in-frame between the DsRED coding sequence and the PEST domain. The fusion gene contains an hCMV promoter and a SV40 polyadenylation site. The BAC was microinjected into the pronuclei of oocytes from FVB/N females. Founder line 12 was established and bred to C57BL/6J mice to generate the colony. Mice were subsequently backcrossed to C57BL/6J mice for three generations. mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression | DsRed is predominantly expressed in the suprachiasmatic nuclei with moderate expression in non-neuronal regions of the cerebral cortex, striatum, cerebellum and dentate gyrus. |
Allele Name | transgene insertion 12, Karl Obrietan |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | mPeriod2-DsRed |
Gene Symbol and Name | Tg(Per2-DsRed*T3)12Obr, transgene insertion 12, Karl Obrietan |
Gene Synonym(s) | |
Promoter | Per2, period circadian clock 2, mouse, laboratory |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | DsRed is predominantly expressed in the suprachiasmatic nuclei with moderate expression in non-neuronal regions of the cerebral cortex, striatum, cerebellum and dentate gyrus. |
Strain of Origin | FVB/N |
Chromosome | UN |
General Note | Three Tg(Per2-DsRed*T3) transgenic lines, nos. 6,8 and 12, exhibit identical patterns of expression in the SCN and other brain regions, differing only in their fluorescence intensity. Data from all three were pooled for qualitative analyses; line 12, which demonstrates the highest expression, was used for quantitative studies. (J:150729) |
Molecular Note | The transgene comprises bacterial artificial chromosome (BAC) #RP24-391L2 containing the mouse period 2 (Per2) gene into which a DsRed.T3-NLS-PEST fusion gene has been inserted. The DsRed.T3 variant contains eight point mutations relative to the parent DsRed gene that increase the protein's maturation kinetics ~10-fold. To improve the protein's kinetic properties further, the PEST domain of the mouse ornithine decarboxylase gene was introduced following the penultimate codon of the gene. A nuclear localization signal (NLS) was cloned in-frame between the DsRed coding sequence and the PEST domain. The sequence encoding the fusion protein is preceded by a human cytomegalovirus (hCMV) promoter and followed by an SV40 polyadenylation signal. Its robust expression in neuronal cells of the suprachiasmatic nuclei (SCN) of the hypothalamus is cyclic and light-inducible; it is also expressed at lower levels in glial cells of brain regions outside the SCN. |
Mutations Made By | Karl Obrietan, Ohio State University |
While maintaining a live colony, these mice are bred as hemizygotes. The donating investigator has not tried to maintain this as a homozygote colony.
When using the B6.FVB-Tg(Per2-DsRed*T3)12Obr/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32839 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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