| Allele Name | transgene insertion 12, Karl Obrietan |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | mPeriod2-DsRed |
| Gene Symbol and Name | Tg(Per2-DsRed*T3)12Obr, transgene insertion 12, Karl Obrietan |
| Gene Synonym(s) | |
| Promoter | Per2, period circadian clock 2, mouse, laboratory |
|---|
| Expressed Gene | RFP, Red Fluorescent Protein, coral |
|---|
| Site of Expression | DsRed is predominantly expressed in the suprachiasmatic nuclei with moderate expression in non-neuronal regions of the cerebral cortex, striatum, cerebellum and dentate gyrus. |
|---|
| Strain of Origin | FVB/N |
| Chromosome | UN |
| General Note | Three Tg(Per2-DsRed*T3) transgenic lines, nos. 6,8 and 12, exhibit identical patterns of expression in the SCN and other brain regions, differing only in their fluorescence intensity. Data from all three were pooled for qualitative analyses; line 12, which demonstrates the highest expression, was used for quantitative studies. (J:150729) |
| Molecular Note | The transgene comprises bacterial artificial chromosome (BAC) #RP24-391L2 containing the mouse period 2 (Per2) gene into which a DsRed.T3-NLS-PEST fusion gene has been inserted. The DsRed.T3 variant contains eight point mutations relative to the parent DsRed gene that increase the protein's maturation kinetics ~10-fold. To improve the protein's kinetic properties further, the PEST domain of the mouse ornithine decarboxylase gene was introduced following the penultimate codon of the gene. A nuclear localization signal (NLS) was cloned in-frame between the DsRed coding sequence and the PEST domain. The sequence encoding the fusion protein is preceded by a human cytomegalovirus (hCMV) promoter and followed by an SV40 polyadenylation signal. Its robust expression in neuronal cells of the suprachiasmatic nuclei (SCN) of the hypothalamus is cyclic and light-inducible; it is also expressed at lower levels in glial cells of brain regions outside the SCN. |
| Mutations Made By | Karl Obrietan, Ohio State University |
