This strain carries a targeted mutation of the Tas1r1 (taste receptor, type 1, member 1) gene which encodes a component of the heterodimeric G-protein coupled umami (monosodium L-glutamate, MSG) taste receptor. Responses to umami L-amino acid tastants are lost in homozygotes. This strain may be useful in studies of taste and nutrient sensing.
Charles Zuker, Columbia University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tas1r1 | taste receptor, type 1, member 1 |
This strain carries a targeted mutation of the Tas1r1 (taste receptor, type 1, member 1) gene which encodes a component of the heterodimeric G-protein coupled umami (monosodium L-glutamate, MSG) taste sensor. Homozygous mice lack expression of the gene and have normal viability, body weight, overall anatomy and general behavior. Taste receptor cells appear normal morphologically and numerically. Although the mice show robust neural responses to sour (citric acid), salty (NaCl), bitter (6-n-propylthiouracil, PROP), and sweet (D-amino acids, sucrose, maltose, glucose, saccharin) tastants, responses to umami L-amino acid tastants are lost. This strain may be useful in studies of taste and nutrient sensing.
Exon 6, encoding the predicted seven transmembrane domain was replaced by a PGK-neomycin resistance cassette in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 for two generations by the donating laboratory.
Allele Name | targeted mutation 1, Charles S Zuker |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Tas1r1, taste receptor, type 1, member 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 4 |
Molecular Note | Exon 6 was replaced with a PGK-neo cassette inserted by homologous recombination. The deleted sequence putatively encoded seven transmembrane domains. Protein was undetected by in situ hybridization of homozygous mutant taste bud sections. |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129-Tas1r1tm1Csz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013064 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Tas1r1<tm1Csz> |
Frozen Mouse Embryo | B6;129-Tas1r1<tm1Csz>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Tas1r1<tm1Csz>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Tas1r1<tm1Csz>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Tas1r1<tm1Csz>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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