B6;129-Tas1r1^tm1Csz/J

Stock number: 013064
Research areas: Neurobiology Research, Sensorineural Research

Description

This strain carries a targeted mutation of the Tas1r1 (taste receptor, type 1, member 1) gene which encodes a component of the heterodimeric G-protein coupled umami (monosodium L-glutamate, MSG) taste receptor. Responses to umami L-amino acid tastants are lost in homozygotes. This strain may be useful in studies of taste and nutrient sensing.

Detailed description

This strain carries a targeted mutation of the Tas1r1 (taste receptor, type 1, member 1) gene which encodes a component of the heterodimeric G-protein coupled umami (monosodium L-glutamate, MSG) taste sensor. Homozygous mice lack expression of the gene and have normal viability, body weight, overall anatomy and general behavior. Taste receptor cells appear normal morphologically and numerically. Although the mice show robust neural responses to sour (citric acid), salty (NaCl), bitter (6-n-propylthiouracil, PROP), and sweet (D-amino acids, sucrose, maltose, glucose, saccharin) tastants, responses to umami L-amino acid tastants are lost. This strain may be useful in studies of taste and nutrient sensing.

Development

Exon 6, encoding the predicted seven transmembrane domain was replaced by a PGK-neomycin resistance cassette in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 for two generations by the donating laboratory.

Allele

Symbol: Tas1r1^tm1Csz
Name: targeted mutation 1, Charles S Zuker
MGI accession ID: MGI:2681885
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tas1r1
Marker name: taste receptor, type 1, member 1
Chromosome: 4
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exon 6 was replaced with a PGK-neo cassette inserted by homologous recombination. The deleted sequence putatively encoded seven transmembrane domains. Protein was undetected by in situ hybridization of homozygous mutant taste bud sections.