Homozygous Ccr6-EGFP mice harbor an enhanced green fluorescent protein (EGFP) "knock-in" allele that both abolishes endogenous Ccr6 gene function and expresses EGFP from the Ccr6 promoter/enhancer elements. These mice exhibit abnormalities in Peyer's patches, decreased isolated lymphoid follicle development in the intestine, and reduced numbers of intestinal M cells.
Ifor Williams, Emory University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Ccr6 | chemokine (C-C motif) receptor 6 |
Homozygous Ccr6-EGFP mice are viable and fertile; harboring an enhanced green fluorescent protein (EGFP) allele that both abolishes endogenous Ccr6 gene function and expresses EGFP from the Ccr6 promoter/enhancer elements. As such, robust EGFP fluorescence is directed to all mature B cells, subpopulations of splenic CD4+ and CD8+ T cells, CD4+ and CD4- myeloid dendritic cells (DC), some CD11c+ DC, and most CD11b+ myeloid DC. Fluorescence is detected at lower levels in epidermal Langerhans cells (LC). These mice exhibit abnormalities in Peyer's patches, decreased isolated lymphoid follicle development in the intestine, and reduced numbers of intestinal M cells.
A targeting vector was designed to replace part of the coding exon of the chemokine (C-C motif) receptor 6 (Ccr6) gene with an enhanced green fluorescent protein (EGFP) sequence followed by a late simian virus 40 (SV40) polyadenylation (polyA) signal. A self-excising loxP-flanked cassette (itself containing a neomycin resistance (Neo) gene and a testes-specific angiotensin-converting enzyme promoter-driven Cre recombinase gene (tACE-Cre)) was inserted 3' to the polyA signal. The construct was electroporated into 129S6/SvEvTac-derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred to C57BL/6J females. These Ccr6-EGFP mice were backcrossed at least 10 generations to C57BL/6J. Upon arrival mice at The Jackson Laboratory these mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP is used to visualize B cell lineage expression patterns. |
Allele Name | targeted mutation 1, Ifor R Williams |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | CCR6-; CCR6-GFP |
Gene Symbol and Name | Ccr6, chemokine (C-C motif) receptor 6 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP is used to visualize B cell lineage expression patterns. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 17 |
Molecular Note | A self-excising cre-neomycin resistance cassette replaced 213 bp of coding sequence at the 5' end of the gene, and placed the open reading frame of EGFP, followed by the late SV40 polyadenylation signal, inframe with upstream sequences. The B cell lineage expression pattern from this allele visualized using EGFP matches that of wild-type gene expression previously described using a mouse-specific monoclonal antibody. |
Mutations Made By | Ifor Williams, Emory University School of Medicine |
When maintaining a live colony, homozygous mice may be bred together.
When using the CCR6-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #013061 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ccr6<tm1(EGFP)Irw> |
Frozen Mouse Embryo | B6.129S6-Ccr6<tm1(EGFP)Irw>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Ccr6<tm1(EGFP)Irw>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Ccr6<tm1(EGFP)Irw>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Ccr6<tm1(EGFP)Irw>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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