B6.129S6-Ccr6^tm1(EGFP)Irw/J

Stock number: 013061
Product name: CCR6-GFP
Strain synonyms: CCR6-
Research areas: Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

Homozygous Ccr6-EGFP mice harbor an enhanced green fluorescent protein (EGFP) "knock-in" allele that both abolishes endogenous Ccr6 gene function and expresses EGFP from the Ccr6 promoter/enhancer elements. These mice exhibit abnormalities in Peyer's patches, decreased isolated lymphoid follicle development in the intestine, and reduced numbers of intestinal M cells.

Detailed description

Homozygous Ccr6-EGFP mice are viable and fertile; harboring an enhanced green fluorescent protein (EGFP) allele that both abolishes endogenous Ccr6 gene function and expresses EGFP from the Ccr6 promoter/enhancer elements. As such, robust EGFP fluorescence is directed to all mature B cells, subpopulations of splenic CD4⁺ and CD8⁺ T cells, CD4⁺ and CD4^- myeloid dendritic cells (DC), some CD11c⁺ DC, and most CD11b⁺ myeloid DC. Fluorescence is detected at lower levels in epidermal Langerhans cells (LC). These mice exhibit abnormalities in Peyer's patches, decreased isolated lymphoid follicle development in the intestine, and reduced numbers of intestinal M cells.

Development

A targeting vector was designed to replace part of the coding exon of the chemokine (C-C motif) receptor 6 (Ccr6) gene with an enhanced green fluorescent protein (EGFP) sequence followed by a late simian virus 40 (SV40) polyadenylation (polyA) signal. A self-excising loxP-flanked cassette (itself containing a neomycin resistance (Neo) gene and a testes-specific angiotensin-converting enzyme promoter-driven Cre recombinase gene (tACE-Cre)) was inserted 3' to the polyA signal. The construct was electroporated into 129S6/SvEvTac-derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred to C57BL/6J females. These Ccr6-EGFP mice were backcrossed at least 10 generations to C57BL/6J. Upon arrival mice at The Jackson Laboratory these mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Ccr6^tm1(EGFP)Irw
Name: targeted mutation 1, Ifor R Williams
MGI accession ID: MGI:2179543
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Ccr6
Marker name: chemokine (C-C motif) receptor 6
Chromosome: 17
Strain of origin: 129S6/SvEvTac
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP is used to visualize B cell lineage expression patterns.
Molecular note: A self-excising cre-neomycin resistance cassette replaced 213 bp of coding sequence at the 5' end of the gene, and placed the open reading frame of EGFP, followed by the late SV40 polyadenylation signal, inframe with upstream sequences. The B cell lineage expression pattern from this allele visualized using EGFP matches that of wild-type gene expression previously described using a mouse-specific monoclonal antibody.