Overview

Also Known As: ER81-CreER

The Etv1-CreERT2 knock-in allele was designed to both abolish ets variant gene 1 (Etv1) gene function and direct CreER^T2 fusion protein expression to ER81 positive neurons (including Layer5 pyramidal neurons) by the endogenous Etv1 promoter/enhancer elements. Cre-ERT2 fusion gene activity is inducible by tamoxifen administration.

Donating Investigator

Z. Josh Huang, Cold Spring Harbor Laboratory

Genetic overview

Genetic Background	Generation
	N11+N1F1 (2018-09-04 00:00:00)

Etv1^{tm1.1(cre/ERT2)Zjh}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing, Inducible)	Etv1	ets variant 1

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Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$278.00 Domestic price for female

356.51 Domestic price for breeder pair

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Details

Detailed Description

The ER81-CreER (or ER81-CreERT2, Etv1-CreER, Etv1-CreERT2) knock-in allele was designed to both abolish ets variant gene 1 (Etv1) gene function and expresses CreER^T2 fusion protein from the Etv1 promoter/enhancer elements. Cre-ERT2 fusion gene activity is inducible; observed following tamoxifen administration. As such, when ER81-CreERT2 mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Etv1-expressing cells of the offspring.

Heterozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Although mice homozygous for other null mutations of this gene on a similar genetic background exhibit neuromuscular abnormalities, ataxia and premature lethality, the donating investigator reports that ER81-CreER homozygous mice show no gross abnormalities. ER81 mRNA or protein expression from the ER81-CreER mutant allele was not determined. The donating investigator also reports tamoxifen-inducible Cre recombinase activity recapitulates the endogenous Etv1 expression pattern, with moderately efficient inducibility. Tamoxifen-inducible Cre recombinase activity is not observed prior to tamoxifen treatment. Following tamoxifen administration, Cre recombinase activity is observed in ER81 positive cortical neurons (including Layer5 pyramidal neurons). The donating investigators did not examine cre expression in tissues other than brain.

For characterization information, see images at the Allen Institute for Brain Science website (Etv1-CreERT2 images).

The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.

Development

A targeting vector was designed to insert a CreER^T2 fusion gene (Cre-ER^T2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the ets variant gene 1 locus (Etv1). This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6 mice (harboring the Tyr^c-2J mutation) to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10); see Stock No. 005703) to remove the neo selection cassette. These ER81-CreER (or ER81-CreERT2, Etv1-CreER, Etv1-CreERT2) mice were subsequently bred to C57BL/6 inbred mice for a few additional generations (and the FLPe transgene was removed) prior to sending to The Jackson Laboratory Repository (see SNP note below). Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the ER81-CreERT2 colony. These ER81-CreERT2 mice may still be segregating at the Tyr locus.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Expression Data

Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Cre recombinase activity is observed in ER81 positive cortical neurons (including Layer5 pyramidal neurons).

Control Suggestions

Wild-type from the colony

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

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Genetics

Etv1^{tm1.1(cre/ERT2)Zjh}

Allele Symbol: Etv1^{tm1.1(cre/ERT2)Zjh}

Allele Name	targeted mutation 1.1, Z Josh Huang
Allele Type	Targeted (Recombinase-expressing, Inducible)
Allele Synonym(s)	Etv1^{tm1.1(cre/ERT2)Zjh}; targeted mutation 1.1, Z Josh Huang
Gene Symbol and Name	Etv1, ets variant 1
Gene Synonym(s)	Etsrp81; ER81; Etsrp81; ets related protein 81
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Cre recombinase activity is observed in ER81 positive cortical neurons (including Layer5 pyramidal neurons).
Strain of Origin	C57BL/6
Chromosome	12
Molecular Note	A targeting vector was designed to insert a Cre/ERT2 fusion gene , an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the ets variant gene 1 locus (Etv1). This construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6 mice (harboring the Tyrc-2J mutation) to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette.
Mutations Made By	Z. Josh Huang, Cold Spring Harbor Laboratory

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Neurobiology Research
  - cell marker
- Genetics Research
  - Tissue/Cell Markers: Cre-lox System
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: neurons
- Cre-lox System
  - Cre Recombinase Expression
  - Cre Recombinase Expression: Inducible
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Etv1^{tm1.1(cre/ERT2)Zjh}/Etv1⁺
involves: 129 * C57BL/6 * SJL

normal phenotype

no abnormal phenotype detected
- Normal - heterozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities

Genotype: Etv1^{tm1.1(cre/ERT2)Zjh}/Etv1^{tm1.1(cre/ERT2)Zjh}
involves: 129 * C57BL/6 * SJL

normal phenotype

no abnormal phenotype detected
- Normal - mice show no gross abnormalities, in contrast to other homozygous mice harboring Etv1 null alleles

References

Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

Additional - Etv1^{tm1.1(cre/ERT2)Zjh} related

Technical Support

Contact Technical Support

Genotyping Protocols
- High Resolution Melting: Etv1^{tm1.1(cre/ERT2)Zjh}
- Standard PCR: Etv1^{tm1.1(cre/ERT2)Zjh}
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J mice (Stock No. 000664). The donating investigator has not fully characterized the homozygous phenotype to date (November 2010).
- Additional Breeding and Husbandry Support
Mating System
- Heterozygote x +/+ sibling
Citation
When using the ER81-CreER mouse strain in a publication, please cite the originating article(s) and include JAX stock #013048 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

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International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Etv1<tm1.1(cre/ERT2)Zjh>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Also Known As: ER81-CreER

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Etv1tm1.1(cre/ERT2)Zjh

Allele Symbol: Etv1tm1.1(cre/ERT2)Zjh

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Etv1tm1.1(cre/ERT2)Zjh/Etv1+involves: 129 * C57BL/6 * SJL

normal phenotype

Genotype: Etv1tm1.1(cre/ERT2)Zjh/Etv1tm1.1(cre/ERT2)Zjhinvolves: 129 * C57BL/6 * SJL

normal phenotype

References

Additional - Etv1tm1.1(cre/ERT2)Zjh related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Etv1^{tm1.1(cre/ERT2)Zjh}

Allele Symbol: Etv1^{tm1.1(cre/ERT2)Zjh}

Genotype: Etv1^{tm1.1(cre/ERT2)Zjh}/Etv1⁺
involves: 129 * C57BL/6 * SJL

Genotype: Etv1^{tm1.1(cre/ERT2)Zjh}/Etv1^{tm1.1(cre/ERT2)Zjh}
involves: 129 * C57BL/6 * SJL

Additional - Etv1^{tm1.1(cre/ERT2)Zjh} related