This mutant mouse strain carries a targeted mutation of the Ftmt, ferritin mitochondrial, mouse gene and may be useful in studies of mitochondrial disease, sideroblastic anemia and iron metabolism.
Mark D Fleming, Children's Hospital Boston
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by RT-PCR analysis of testes from homozygotes. Homozygotes do not exhibit any overt phenotype.
A targeting vector containing a neomycin selection cassette was used to disrupt the entire open-reading frame fo the gene. The construct was electroporated into 129S4/SvJae derived-J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6J for 17 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, Mark D Fleming|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Ftmt, ferritin mitochondrial|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A targeting vector containing a neomycin selection cassette was used to disrupt the entire open-reading frame fo the gene. No gene product (mRNA) is detected by RT-PCR analysis of testes from homozygotes.|
|Mutations Made By|| |
Mark Fleming, Children's Hospital Boston
When maintaining a live colony, these mice can be bred as homozygotes
When using the B6.129S4-Ftmttm1Mdf/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012932 in your Materials and Methods section.