These Egr1 knock-out mice may be useful for studying carcinogenesis, inflammation, atherosclerosis, scleroderma, ischemic injury, tissue repair, fibrosis, matrix remodeling and wound healing.
Jeffrey Milbrandt, Washington University School of Medicine, St. Louis
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Egr1 | early growth response 1 |
In this strain a coding exon upstream of the DNA-binding domain of the early growth response 1 (Egr1) gene is replaced with a neo cassette, abolishing gene function. Homozygous Egr1-/- mice are viable and normal in size. Homozygous females are sterile, are absent of corpora lutea, and display a 30% reduction in the weight of the uterus. These mice exhibit an impaired inflammatory response, impaired wound healing, and attenuated dermal fibrosis after TGF-β or bleomycin stimulation. Macrophages from Egr1-/- mice showed reduced TNF-α secretion when stimulated with TGF-β. These mice may be useful for studying carcinogenesis, inflammation, atherosclerosis, scleroderma, ischemic injury, tissue repair, fibrosis, matrix remodeling and wound healing.
A targeting vector was designed to place a neomycin resistance (neo) cassette into a coding exon upstream of the DNA-binding domain of the early growth response 1 (Egr1) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6NTac females to generate a colony of Egr1-/- mice. The Donating investigator confirms that these mice were backcrossed to C57BL/6NTac mice for 26 generations (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 4 markers on 3 chromosomes, 6, 12, and 13, were found to be segregating, suggesting an incomplete backcross.
Allele Name | targeted mutation 1, Jeffrey Milbrandt |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | egr-1-; Egr1-; Egr-10; Egr-1ko; NGFI-A- |
Gene Symbol and Name | Egr1, early growth response 1 |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 18 |
General Note | ES cell line = AB1 (129S7/SvEvBrd-Hprt+) or D3 (129S2/SvPas). |
Molecular Note | The gene was disrupted by insertion of a PGK-neo cassette into a coding exon upstream of the DNA-binding domain via homologous recombination. The targeted mutation introduces several stop codons resulting protein truncation upstream of the DNA-binding domain. Gene expression and protein product were not observed in homozygous mutant ES cells. |
Mutations Made By | Jeffrey Milbrandt, Washington University School of Medicine, St. Louis |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony. Homozygous females are sterile.
When using the NGFI-A mouse strain in a publication, please cite the originating article(s) and include JAX stock #012924 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Egr1<tm1Jmi> |
Frozen Mouse Embryo | B6N;129-Egr1<tm1Jmi>/J | $2595.00 |
Frozen Mouse Embryo | B6N;129-Egr1<tm1Jmi>/J | $2595.00 |
Frozen Mouse Embryo | B6N;129-Egr1<tm1Jmi>/J | $3373.50 |
Frozen Mouse Embryo | B6N;129-Egr1<tm1Jmi>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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