These Egr1 knock-out mice may be useful for studying carcinogenesis, inflammation, atherosclerosis, scleroderma, ischemic injury, tissue repair, fibrosis, matrix remodeling and wound healing.
Jeffrey Milbrandt, Washington University in St. Louis
In this strain a coding exon upstream of the DNA-binding domain of the early growth response 1 (Egr1) gene is replaced with a neo cassette, abolishing gene function. Homozygous Egr1-/- mice are viable and normal in size. Homozygous females are sterile, are absent of corpora lutea, and display a 30% reduction in the weight of the uterus. These mice exhibit an impaired inflammatory response, impaired wound healing, and attenuated dermal fibrosis after TGF-β or bleomycin stimulation. Macrophages from Egr1-/- mice showed reduced TNF-α secretion when stimulated with TGF-β. These mice may be useful for studying carcinogenesis, inflammation, atherosclerosis, scleroderma, ischemic injury, tissue repair, fibrosis, matrix remodeling and wound healing.
A targeting vector was designed to place a neomycin resistance (neo) cassette into a coding exon upstream of the DNA-binding domain of the early growth response 1 (Egr1) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6NTac females to generate a colony of Egr1-/- mice. The Donating investigator confirms that these mice were backcrossed to C57BL/6NTac mice for 26 generations (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 4 markers on 3 chromosomes, 6, 12, and 13, were found to be segregating, suggesting an incomplete backcross.
|Allele Name||targeted mutation 1, Jeffrey Milbrandt|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Egr-10; Egr-1ko; Egr1-; NGFI-A-; egr-1-|
|Gene Symbol and Name||Egr1, early growth response 1|
|Gene Synonym(s)||A530045N19Rik; A530045N19Rik; AT225; ETR103; Egr-1; Egr-1; G0S30; KROX-24; Krox-1; Krox-1; Krox-24; Krox-24; Krox24; Kruppel box 1; Kruppel box 24; NGF1-A; NGFI-A; NGFIA; Ngf1; Ngfi; RIKEN cDNA A530045N19 gene; TIS8; ZIF-268; ZNF225; Zenk; Zfp-6; Zfp-6; Zif268; zif-268; zinc finger protein 6|
|Strain of Origin||129|
|General Note||ES cell line = AB1 (129S7/SvEvBrd-Hprt+) or D3 (129S2/SvPas). Phenotypic Similarity to Human Syndrome: Myeloproliferative disorder J:123870 background involves: 129 * C57BL/6|
|Molecular Note||The gene was disrupted by insertion of a PGK-neo cassette into a coding exon upstream of the DNA-binding domain via homologous recombination. The targeted mutation introduces several stop codons resulting protein truncation upstream of the DNA-binding domain. Gene expression and protein product were not observed in homozygous mutant ES cells.|
|Mutations Made By|| |
Jeffrey Milbrandt, Washington University in St. Louis
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony. Homozygous females are sterile.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
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