Homozyous Bpnt2 (3'(2'), 5'-bisphosphate nucleotidase 2; also called gPAPP) mutant mice die within 10 minutes of birth due to severe respiratory insufficiency and chondrodysplasia. Mutants have shortened limbs due to defects in endochondral ossification which are hypothesized to be due to an undersulfonation of chondroitin and heparin sulfate. This strain may be useful in studies of skeletal development and sulfation.
John York, Duke University Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Reporter, Null/Knockout) | Bpnt2 | 3'(2'), 5'-bisphosphate nucleotidase 2 |
Homozyous Bpnt2 (3'(2'), 5'-bisphosphate nucleotidase 2; also called gPAPP) mutant mice die within 10 minutes of birth due to severe respiratory insufficiency and chondrodysplasia. Mutants have shortened limbs due to defects in endochondral ossification which are hypothesized to be due to an undersulfonation of chondroitin and heparin sulfate. No phenotypic differences have been observed between this C57BL/6 background strain and the mixed C57BL/6-129 background strain (Stock No. 012921). This strain may be useful in studies of skeletal development and sulfation.
A 129P2/OlaHsd-derived mouse embryonic stem (ES) cell line (RST 634, Bay Genomics) carrying only the first exon of the targeted gene fused to beta galactosidase (lacZ) was used to create this strain. This strain was backcrossed to C57BL/6 for 8 generations by the donating laboratory.
Allele Name | gene trap RST634, BayGenomics |
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Allele Type | Gene trapped (Reporter, Null/Knockout) |
Allele Synonym(s) | gPAPPgt |
Gene Symbol and Name | Bpnt2, 3'(2'), 5'-bisphosphate nucleotidase 2 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 4 |
Molecular Note | Insertion of the gene-trap vector in the first intron encodes the first exon of Impad1 gene fused to beta-galactosidase. It is predicted to express a truncated mutant protein that would contain only 1 of 13 catalytically conserved residues. Western blot and biochemical analyses confirmed homozygous mice are deficient for functional enzyme. |
When maintained as a live colony, heterozygotes may be bred. Homozygotes die shortly after birth.
When using the B6.129P2-Bpnt2Gt(RST634)Byg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012922 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Impad1<Gt(RST634)Byg> |
Frozen Mouse Embryo | B6.129P2-Impad1<Gt(RST634)Byg>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Impad1<Gt(RST634)Byg>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Impad1<Gt(RST634)Byg>/J | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Impad1<Gt(RST634)Byg>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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