These floxed mutant mice possess loxP sites flanking exon 2 of the Nr3c1 gene. This strain may be useful for generating conditional mutations in applications related to hypothalamic-pituitary-adrenal axis homeostasis.
Louis J Muglia, Cincinnati Children's Hospital Medl Ctr
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).
When bred to a strain with Cre recombinase expression in the T cell lineage (see Stock No. 003802 for example), this mutant mouse strain may be useful in studies of hypothalamic-pituitary-adrenal axis homeostasis.
A loxP site flanked targeting vector containing PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 2 of the targeted gene on chromosome 18, and another loxP site was inserted upstream of exon 1C. This construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to transgenic mice (on the C57BL/6 genetic background) expressing Cre recombinase under the control of the mouse, Lck, lymphocyte protein tyrosine kinase, promoter. The donating investigator reported that mice that retained the loxP site flanked exon 2 were then bred to C57BL/6 mice (see SNP note below) for 10 generations. These mice no longer carry the PGK-Neo selection cassette. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N was found to be segregating (the marker on chromosome 11). Of note, this chromosome 11 marker is the same for C57BL/6N and 129S. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 2.1, Louis J Muglia|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||GrloxP; Nr3c1flox; Nr3c1flox|
|Gene Symbol and Name||Nr3c1, nuclear receptor subfamily 3, group C, member 1|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Cre mediated recombination removed the neo cassette leaving exons 1c through exon 2 floxed.|
|Mutations Made By|| |
Louis Muglia, Vanderbilt University School of Medicine
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Nr3c1flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #012914 in your Materials and Methods section.