B6;129S-Stat1^tm1Mam/Mmjax

Stock number: 012901
Research areas: Research Tools

Description

These Stat1^fl (signal transducer and activator of transcription 1) mutant mice may be useful in generating conditional mutations to study autonomous roles of STAT1 in ERBB2/neu induced mammary tumorigenesis.

Detailed description

Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene. This mutant mouse strain may be useful in generating conditional mutations to study autonomous roles of STAT1 in ERBB2/neu induced mammary tumorigenesis.

Heterozygote: Not evaluated

Development

A targeting vector was designed to place a loxP flanked neomycin resistance gene upstream of the first untranslated exon followed by a loxP site downstream of the first translated exon. The construct was electroporated into 129S/SvEv derived embryonic stem (ES) cells. Transient Cre expression in targeted cells excised the neo cassette leaving loxP sites flanking the first two untranslated exons and the first translated exon. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and maintained as sibling matings. Upon arrival, mice were bred to C57BL/6 for at least 1 generation to establish the colony.

Allele

Symbol: Stat1^tm1Mam
Name: targeted mutation 1, Lothar Hennighausen
MGI accession ID: MGI:4821871
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Stat1
Marker name: signal transducer and activator of transcription 1
Chromosome: 1
Strain of origin: 129S/SvEv
Molecular note: A targeting vector was designed to place a loxP flanked neomycin resistance gene upstream of the first untranslated exon followed by a loxP site downstream of the first translated exon. The neo cassette was removed by germ-line cre-mediated resombination leaving loxP sites flanking the first two untranslated exons and the first translated exon.