These Stat1fl (signal transducer and activator of transcription 1) mutant mice may be useful in generating conditional mutations to study autonomous roles of STAT1 in ERBB2/neu induced mammary tumorigenesis.
Dr. Lothar Hennighausen, National Institutes of Health
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Stat1 | signal transducer and activator of transcription 1 |
Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene. This mutant mouse strain may be useful in generating conditional mutations to study autonomous roles of STAT1 in ERBB2/neu induced mammary tumorigenesis.
Heterozygote: Not evaluated
A targeting vector was designed to place a loxP flanked neomycin resistance gene upstream of the first untranslated exon followed by a loxP site downstream of the first translated exon. The construct was electroporated into 129S/SvEv derived embryonic stem (ES) cells. Transient Cre expression in targeted cells excised the neo cassette leaving loxP sites flanking the first two untranslated exons and the first translated exon. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and maintained as sibling matings. Upon arrival, mice were bred to C57BL/6 for at least 1 generation to establish the colony.
Allele Name | targeted mutation 1, Lothar Hennighausen |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Stat1 fl/fl |
Gene Symbol and Name | Stat1, signal transducer and activator of transcription 1 |
Gene Synonym(s) | |
Strain of Origin | 129S/SvEv |
Chromosome | 1 |
Molecular Note | A targeting vector was designed to place a loxP flanked neomycin resistance gene upstream of the first untranslated exon followed by a loxP site downstream of the first translated exon. The neo cassette was removed by germ-line cre-mediated resombination leaving loxP sites flanking the first two untranslated exons and the first translated exon. |
Mutations Made By | Dr. Lothar Hennighausen, National Institutes of Health |
While maintaining a live colony, these mice are bred as homozygotes.
When using the B6;129S-Stat1tm1Mam/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32054 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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