These Stat1fl (signal transducer and activator of transcription 1) mutant mice may be useful in generating conditional mutations to study autonomous roles of STAT1 in ERBB2/neu induced mammary tumorigenesis.
Dr. Lothar Hennighausen, National Institutes of Health
Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene. This mutant mouse strain may be useful in generating conditional mutations to study autonomous roles of STAT1 in ERBB2/neu induced mammary tumorigenesis.
Heterozygote: Not evaluated
A targeting vector was designed to place a loxP flanked neomycin resistance gene upstream of the first untranslated exon followed by a loxP site downstream of the first translated exon. The construct was electroporated into 129S/SvEv derived embryonic stem (ES) cells. Transient Cre expression in targeted cells excised the neo cassette leaving loxP sites flanking the first two untranslated exons and the first translated exon. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and maintained as sibling matings. Upon arrival, mice were bred to C57BL/6 for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Lothar Hennighausen|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||targeted mutation 1, Lothar Hennighausen; Stat1tm1Mam|
|Gene Symbol and Name||Stat1, signal transducer and activator of transcription 1|
|Gene Synonym(s)||RIKEN cDNA 2010005J02 gene; 2010005J02Rik; 2010005J02Rik; AA408197; expressed sequence AA408197; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91; CANDF7; DD6G4-4|
|Strain of Origin||129S/SvEv|
|Molecular Note||A targeting vector was designed to place a loxP flanked neomycin resistance gene upstream of the first untranslated exon followed by a loxP site downstream of the first translated exon. The neo cassette was removed by germ-line cre-mediated resombination leaving loxP sites flanking the first two untranslated exons and the first translated exon.|
|Mutations Made By|| |
Dr. Lothar Hennighausen, National Institutes of Health
While maintaining a live colony, these mice are bred as homozygotes.
When using the B6;129S-Stat1tm1Mam/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32054 in your Materials and Methods section.
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