Overview

This strain is undergoing recovery from cryopreserved stocks.

GFAP-Cre transgenic mice from founder line 73.12 have Cre recombinase expression directed by the mouse glial fibrillary acidic protein promoter. Cre expression is observed in astrocytes in the brain and spinal cord, as well as postnatal and adult GFAP-expressing neural stem cells and their progeny in the brain.

Donating Investigator

Michael V Sofroniew, University of California Los Angeles

Genetic overview

Genetic Background	Generation
	N14+pN1F4 (2021-04-06 00:00:00)

Tg(Gfap-cre)73.12Mvs

Allele Type
Transgenic (Recombinase-expressing)

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Research Applications

Research Tools
Developmental Biology Research
Neurobiology Research

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Base Price

Starting at:

$255.00 Domestic price for female 4-week

333.51 Domestic price for breeder pair

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Details

Detailed Description

Mice hemizygous for the GFAP-cre transgene are viable and fertile, with a mouse glial fibrillary acidic protein (mGfap) promoter sequence directing expression of Cre recombinase. Specifically, Cre recombinase activity (as defined by expression of a floxed-STOP reporter gene) is targeted to most astrocytes throughout healthy brain and spinal cord tissues, and to essentially all astrocytes following central nervous system (CNS) injury.

In contrast to GFAP-Cre line 77.6 (Stock No. 024098), these GFAP-Cre line 73.12 mice (Stock No. 012886) are reported to have Cre recombinase activity in essentially all adult neural stem cells (and their progeny) from the hippocampal dentate gyrus and subventricular zone. This includes dentate gyrus granule neurons and their mossy fiber axon projections into the CA3 region of the hippocampus. In addition, GFAP-Cre line 73.12 mice exhibit Cre recombinase activity in some hippocampal pyramidal neurons and cerebellar granule neurons (less than 5%), and scattered neurons in the midbrain (less than 0.5%): this is attributed to radial cell progenitors that begin to express Gfap at later developmental stages in postnatal mice when the last-born neurons are generated. Additionally, the donating investigator reports GFAP-Cre lines 73.12 and 77.6 have cre expression in the male germline, and suggests breeding GFAP-Cre females with floxed males for Cre-lox experiments.

When GFAP-Cre line 73.12 mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These GFAP-Cre line 73.12 mice may be useful for studying astrocytes in the brain and spinal cord, as well as postnatal and adult GFAP-expressing neural stem cells and their progeny in the brain.

Development

The GFAP-Cre transgene was designed with the entire mouse glial fibrillary acidic protein gene (Gfap) sequences directing expression of Cre recombinase. The construct has a small fragment of the Gfap first exon removed to prevent GFAP expression from the transgene. The transgene was microinjected into fertilized (BALB/c x C57BL/6NHsd)F1 oocytes. Transgenic founders were bred to C57BL/6 mice, and founder line 73.12 was identified. The GFAP-Cre line 73.12 colony was backcrossed to C57BL/6 animals (see SNP note below) for at least 14 generations, and then sent to The Jackson Laboratory Repository in 2010. Upon arrival, males were used to cryopreserve sperm. To establish the living mouse colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J inbred mice (Stock No. 000664).

In 2010, a 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
In 2019, our live colony was rederived using cryopreserved sperm (originally frozen in 2010) to fertilize C57BL/6J oocytes. The resulting first generation rederived living colony was segregating for the markers that determine C57BL/6J from C57BL/6N. This is expected and supports the previous SNP results.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed in essentially all adult neural stem cells and their progeny in the hippocampal dentate gyrus and subventricular zone. In addition, some hippocampal pyramidal neurons and cerebellar granule neurons (less than 5%) and scattered neurons in the midbrain (less than 0.5%) also exhibit activity.

Control Suggestions

Noncarrier

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Garcia AD; Doan NB; Imura T; Bush TG; Sofroniew MV. 2004. GFAP-expressing progenitors are the principal source of constitutive neurogenesis in adult mouse forebrain. Nat Neurosci 7(11):1233-41PubMed: 15494728 MGI: J:94543

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Genetics

Tg(Gfap-cre)73.12Mvs

Allele Symbol: Tg(Gfap-cre)73.12Mvs

Allele Name	transgene insertion 73.12, Michael V Sofroniew
Allele Type	Transgenic (Recombinase-expressing)
Allele Synonym(s)	GFAP-Cre; mGFAP^Cre; mGFAP-Cre
Gene Symbol and Name	Tg(Gfap-cre)73.12Mvs, transgene insertion 73.12, Michael V Sofroniew
Gene Synonym(s)
Promoter	Gfap, glial fibrillary acidic protein, mouse, laboratory
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed in essentially all adult neural stem cells and their progeny in the hippocampal dentate gyrus and subventricular zone. In addition, some hippocampal pyramidal neurons and cerebellar granule neurons (less than 5%) and scattered neurons in the midbrain (less than 0.5%) also exhibit activity.
Strain of Origin	(BALB/c x C57BL/6NHsd)F1
Chromosome	UN
Molecular Note	The bacterial Cre recombinase gene was inserted into the first exon of an expression cassette (clone 445) that contains all the introns, promoter regulatory elements, exons, and 2.5 kb of 5' and 2 kb of 3' flanking DNA of the astroglia-specific mouse glial fibrillary acidic protein gene. GFAP is not expressed from the transgene due to deletion of part of exon 1.
Mutations Made By	Michael Sofroniew, University of California Los Angeles

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Tissue/Cell Markers
  - Tissue/Cell Markers: astrocytes
  - Tissue/Cell Markers: astrocytes, neurons
- Cre-lox System
  - Cre Recombinase Expression
Developmental Biology Research
- Internal/Organ Defects
  - brain
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

References

Garcia AD; Doan NB; Imura T; Bush TG; Sofroniew MV. 2004. GFAP-expressing progenitors are the principal source of constitutive neurogenesis in adult mouse forebrain. Nat Neurosci 7(11):1233-41PubMed: 15494728 MGI: J:94543

Additional - Tg(Gfap-cre)73.12Mvs related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- QPCR:Generic Cre Quantitative PCR
- Separated PCR:Tg(Gfap-cre)73.12Mvs
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together. Of note, the donating investigator reports GFAP-Cre lines 73.12 and 77.6 have cre expression in the male germline, and suggests breeding GFAP-Cre females with floxed males for Cre-lox experiments.
- Additional Breeding and Husbandry Support
Mating System
- Hemizygote x Noncarrier
- Noncarrier x Hemizygote
Citation
When using the B6.Cg-Tg(Gfap-cre)73.12Mvs/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012886 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

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International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Hemizygous or Non carrier for Tg(Gfap-cre)73.12Mvs

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Tg(Gfap-cre)73.12Mvs

Allele Symbol: Tg(Gfap-cre)73.12Mvs

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Tg(Gfap-cre)73.12Mvs related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information