Mice homozygous for the quivery runt mutation can be identified by three weeks of age by their runted appearance and quivering movement. The symptoms progress rapidly and homozygotes can die anytime after wean. Only a few have survived to ten weeks of age. Hearing loss has been found in some, but not all, homozygotes at 3 and 3.5 weeks of age.
The quivery runt mutation was found in the progeny of an ENU treated C57BL/6J male. As part of a mutagenesis screen this mutagenized male was crossed to C3Fe.Cg-Rw and their offspring were bred to C3Fe.Cg-Hm +/+ Rw to identify mutations mapping to the rump white deletion region. Quivery runt did not map to the rump white deletion region, but instead to Chromosome 14. To ensure removal of any additional ENU-induced mutations this mutant line was maintained by backcrossing to C57BL/6J via homozygous ovarian transplant x C57BL/6J then obligate intercross. Sperm was cryopreserved from heterozygous males at generation N6.
Because of the severity of the phenotype and premature death this strain is maintained by breeding homozygous ovarian transplant hosts with C57BL/6J males and then intercrossing the obligate heterozygous offspring.
When using the B6(Cg)-qvyr/GrsrJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #012875 in your Materials and Methods section.