Mice homozygous for the Map3k11m1J mutation can be identified easily as early as 3 to 4 days of age by the dorsal lines of dark red skin that run from head to tail along the spine and from left to right across the crown of the head, base of the neck in front of the shoulders, and between the base of the ribs and the pelvis. These lines fade away and by 3 weeks of age are no longer evident even when the homozygote is shaved to expose the skin. Homozygotes have necrotic dental pulp, which also improves with age.
The Map3k11m1J mutation arose spontaneously in the STOCK Bdnftm3Jae/J strain at The Jackson Laboratory in 2004. This mutant subline was maintained by sibling inbreeding for many generations before being outcrossed for two consecutive generations to BALB/cByJ and then maintained by intercross selecting out the Bdnftm3Jae mutation. This mutant subline reached generation N2F2 in 2011.
|Allele Name||mutation 1 Jackson|
|Gene Symbol and Name||Map3k11, mitogen-activated protein kinase kinase kinase 11|
|Strain of Origin||STOCK Bdnftm3Jae/J|
|Molecular Note||A spontaneous G-to-A mutation at position 5,695,295 (GRCm38) changes amino acid 293 from glutamate to lysine (p.E293K).|