These C57BL/6-plt mice harbor the spontaneous plt (or "paucity of lymph node T cells") deletion of the Ccl19 and Ccl21a loci on chromosome 4. Lack of expression of these two CCR7 receptor ligands in the secondary lymphoid organs results in abnormal leukocyte migration and impaired immune response. These mutant mice may be useful in studying leukocyte migration into lymphatic tissues, elucidating differences between T cell and B cell trafficking, and the role of stromal cells in bringing naive T cells and dendritic cells together for the initiation of immune responses.
Hideki Nakano, NIEHS/NIH
Homozygous C57BL/6-plt mice are viable and fertile, harboring the spontaneous plt (or "paucity of lymph node T cells") deletion of both the Ccl19 and Ccl21a loci on chromosome 4. Lack of expression of these two CCR7 ligands in the secondary lymphoid organs results in abnormal leukocyte migration and impaired immune response. Specifically, homozygous mice have disrupted trafficking/homing of T cells and dendritic cells to lymphoid tissues. No reported abnormalities in B cell distribution/cellularity are reported for homozygous mice. Transient migration of antigen-stimulated B cells in lymphoid organs (which facilitates B cell/helper T cell interactions) is also impaired in homozygous mice. Homozygous mice exhibit mature single-positive thymocyte arrest in the thymic cortex that results in defective formation of the medullary region of the thymus. Thymic export of T cells in these mice is compromised during the neonatal period but not in adulthood. While CCL21 expression is absent in lymphoid organs because of the Ccl21a deletion, CCL21 expression in non-lymphoid organs is observed because the upstream Cc121b locus remains intact. These C57BL/6-plt mice may be useful in studying leukocyte migration into lymphatic tissues, elucidating differences between T cell and B cell trafficking, and the role of stromal cells in bringing naive T cells and dendritic cells together for the initiation of immune responses.
The donating investigator provided these C57BL/6-plt mice, as well as plt mice congenic on a BALB/c genetic background (BALB/c-plt ; Stock No. 012873). The donating investigator reports dendritic cell migration is severely impaired in both homozygous strains. In addition, they report T cell homing to lymph nodes is severely impaired in BALB/c-plt homozygotes, but mildly impaired in C57BL/6-plt homozygotes. BALB/c-plt homozygous mice have been shown to allow enhanced survival of MHC-mismatched islet allografts (this phenotype is not characterized for C57BL/6-plt mice)[July 2010]).
In 1997, Dr. Hideki Nakano (while at the Laboratory Animal Research Center, University of Tokyo, Japan) reported that their colony of DDD/1 inbred mice exhibited greatly diminished T cell numbers in lymph nodes. This spontaneous deletion, designated plt (or "paucity of lymph node T cells"), behaves as a single autosomal recessive allele and deletes a portion of chromosome 4 including both the Ccl19 (Epstein-Barr virus-induced molecule-1 ligand chemokine [ELC], ELC-atg, or Scya19) and Ccl21a (6CKine-ser, 6CKBAC2, secondary lymphoid organ chemokine [SLC], SLC-Ser) loci. Some plt-mutant mice were backcrossed to C57BL/6NCrl for eleven generations to generate the C57BL6-plt congenic strain. Dr. Hideki Nakano (while at NIEHS/NIH, Research Triangle Park. North Carolina USA) sent homozygous C57BL6-plt females and males to The Jackson Laboratory Repository in 2010. Upon arrival, C57BL6-plt mice were bred together to maintain the colony. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6NCrl genetic background.
The donating investigator reports that C57BL6-plt mice should have retained the plt haplotype at the Chemokine Locus on Mouse Chromosome 4 (Cklc4plt). Thus the genomic organization of Cklc4 would be the same as DDD/1-plt mice: Ccl21b intact and functional (Ccl21a deleted), Scya19-ps1 (Ccl19-ps1) intact (Scya19 [Ccl19] deleted), Scya27a (Ccl27a) intact and functional, and Il11ra1 intact and functional. The Jackson Laboratory will verify these mice harbor the plt deletion, but will not extensively verify the genetic composition of the other loci described for the Cklc4plt haplotype. The Cklc4plt haplotype will need to be verified by the investigator using the mice if necessary.
|Allele Name||paucity of lymph node T-cells|
|Gene Symbol and Name||plt, paucity of lymph node T cells|
|Strain of Origin||DDD/1|
|Molecular Note||This mutation is a deletion region on chromosome 4 which includes the Ccl19 and Ccl21b genes (see also revised nomenclature for the Ccl21 family of genes).|
|Mutations Made By|| |
Hideki Nakano, NIEHS/NIH
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.