B6.129X1-Ccl19^tm1Cys/J

Stock number: 012851
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Homozygous Ccl19 (chemokine (C-C motif) ligand 19) targeted mutation mice are born at predicted Mendelian ratios, are fertile and appear healthy. They lack detectable mRNA and protein in secondary lymphoid organs and show mild alterations in T cell homeostasis.

Detailed description

Homozygous Ccl19 (chemokine (C-C motif) ligand 19) targeted mutation mice are born at predicted Mendelian ratios, are fertile and appear healthy. They lack detectable mRNA and protein in secondary lymphoid organs and show mild alterations in T cell homeostasis. Although present in the targeting construct, GFP is not expressed and can't be detected by any method.

Development

Nucleotides 38-120 of exon 2 were replaced with an in-frame stop codon followed by a Mengo virus internal ribosomal entry site and the gene encoding green fluorescent protein (GFP) as well as a loxP-flanked neomycin-resistance gene. The mutation was created in 129X1/SvJ-derived JM-1 embryonic stem (ES) cells. The neomycin cassette was excised through a cross with a beta actin promoter cre recombinase strain (C57BL/6 (backcross N3) background). This strain was then backcrossed to C57BL/6 for eleven generations by the donating laboratory.

Allele

Symbol: Ccl19^tm1Cys
Name: targeted mutation 1, Jason G Cyster
MGI accession ID: MGI:3762257
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Ccl19
Marker name: chemokine (C-C motif) ligand 19
Chromosome: 4
Strain of origin: 129X1/SvJ
Molecular note: Nucleotides 38 through 120 of the second exon were replaced with a stop codon followed by an IRES-EGFP and a floxed neo cassette. The neo cassette was subsequently removed by cre-mediated recombination following a cross to mice carrying the cre gene driven by a beta-actin promoter (allele not specified). The absence of protein product was confirmed by western blot analysis on proteins extracted from the lymph nodes and spleen.
General note: The gene targeting removed one CCL19 genes (the one with an atg start codon; CCL19a) but not the transcribed pseudogenes of CCL19 (acg start codon; CCL19b, CCL19c). Most or all CCL19 protein is gone in the mutant mice.