Homozygous Ccl19 (chemokine (C-C motif) ligand 19) targeted mutation mice are born at predicted Mendelian ratios, are fertile and appear healthy. They lack detectable mRNA and protein in secondary lymphoid organs and show mild alterations in T cell homeostasis.
Jason Cyster, University of California San Francisco
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Ccl19 | chemokine (C-C motif) ligand 19 |
Homozygous Ccl19 (chemokine (C-C motif) ligand 19) targeted mutation mice are born at predicted Mendelian ratios, are fertile and appear healthy. They lack detectable mRNA and protein in secondary lymphoid organs and show mild alterations in T cell homeostasis. Although present in the targeting construct, GFP is not expressed and can't be detected by any method.
Nucleotides 38-120 of exon 2 were replaced with an in-frame stop codon followed by a Mengo virus internal ribosomal entry site and the gene encoding green fluorescent protein (GFP) as well as a loxP-flanked neomycin-resistance gene. The mutation was created in 129X1/SvJ-derived JM-1 embryonic stem (ES) cells. The neomycin cassette was excised through a cross with a beta actin promoter cre recombinase strain (C57BL/6 (backcross N3) background). This strain was then backcrossed to C57BL/6 for eleven generations by the donating laboratory.
Allele Name | targeted mutation 1, Jason G Cyster |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Ccl19-; Ccl19tm1Salu |
Gene Symbol and Name | Ccl19, chemokine (C-C motif) ligand 19 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 4 |
General Note | The gene targeting removed one CCL19 genes (the one with an atg start codon; CCL19a) but not the transcribed pseudogenes of CCL19 (acg start codon; CCL19b, CCL19c). Most or all CCL19 protein is gone in the mutant mice. |
Molecular Note | Nucleotides 38 through 120 of the second exon were replaced with a stop codon followed by an IRES-EGFP and a floxed neo cassette. The neo cassette was subsequently removed by cre-mediated recombination following a cross to mice carrying the cre gene driven by a beta-actin promoter (allele not specified). The absence of protein product was confirmed by western blot analysis on proteins extracted from the lymph nodes and spleen. |
Mutations Made By | Jason Cyster, University of California San Francisco |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129X1-Ccl19tm1Cys/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012851 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Ccl19<tm1Cys> |
Frozen Mouse Embryo | B6.129X1-Ccl19<tm1Cys>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Ccl19<tm1Cys>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Ccl19<tm1Cys>/J | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Ccl19<tm1Cys>/J | $3373.50 |
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