STOCK Tg(Ggt1-cre)M3Egn/J

Stock number: 012841
Research areas: Research Tools

Description

These GT.cre transgenic mice express Cre recombinase in cortical tubular epithelium of the kidney and may be useful for generating conditional mutations to study polycystic kidney disease and renal fibrosis.

Detailed description

These transgenic mice express Cre recombinase under the control of the rat Ggt1, gamma-glutamyltransferase 1, promoter. Cre recombinase expression is detected by Northern blot in the kidney beginning at age 7 days. No transcript was detected in brain, liver, spleen, muscle, lung or adrenal gland. Cre recombinase protein is detected immunohistochemically in cortical proximal tubules. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the cortical tubular epithelium. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that homozygotes are viable and fertile.

Development

A transgenic construct containing sequence encoding cre recombinase under the control of a rat Ggt1 (gamma-glutamyltransferase 1) promoter and a human growth hormone polyadenylation site, was introduced into B6 X SJL eggs. The resulting founder line, M3 mice, were crossed to BALB/c mice. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J for at least one generation to establish the colony.

In 2011, a 32 SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that this strain included a mix of C57BL/6 substrains (40-80% C57BL/6N). By 2016, the genetic background is likely to be a mix of BALB/cJ and C57BL/6 (expected coat color is agouti).

Allele

Symbol: Tg(Ggt1-cre)M3Egn
Name: transgene insertion M3, Eric G Neilson
MGI accession ID: MGI:3811269
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Ggt1-cre)M3Egn
Marker name: transgene insertion M3, Eric G Neilson
Chromosome: UN
Strain of origin: C57BL/6 x SJL
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre expression is limited to cortical tubular epithelium of the kidney, and is first detected around 7 days of age.
Molecular note: The rat proximal promoter to Ggt1 was used in a transgene construct to drive the expression of cre recombinase, followed by a termination sequence from human growth hormone. Cre expression was limited to cortical tubular epithelium of the kidney, and was first detected around 7 days of age.