These GT.cre transgenic mice express Cre recombinase in cortical tubular epithelium of the kidney and may be useful for generating conditional mutations to study polycystic kidney disease and renal fibrosis.
Eric G. Neilson, Vanderbilt University
Genetic Background | Generation |
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?+pN1F11
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Allele Type |
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Transgenic (Recombinase-expressing) |
These transgenic mice express Cre recombinase under the control of the rat Ggt1, gamma-glutamyltransferase 1, promoter. Cre recombinase expression is detected by Northern blot in the kidney beginning at age 7 days. No transcript was detected in brain, liver, spleen, muscle, lung or adrenal gland. Cre recombinase protein is detected immunohistochemically in cortical proximal tubules. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the cortical tubular epithelium. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that homozygotes are viable and fertile.
A transgenic construct containing sequence encoding cre recombinase under the control of a rat Ggt1 (gamma-glutamyltransferase 1) promoter and a human
growth hormone polyadenylation site, was introduced into B6 X SJL eggs. The resulting founder line, M3 mice, were crossed to BALB/c mice. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J for at least one generation to establish the colony.
In 2011, a 32 SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that this strain included a mix of C57BL/6 substrains (40-80% C57BL/6N). By 2016, the genetic background is likely to be a mix of BALB/cJ and C57BL/6 (expected coat color is agouti).
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre expression is limited to cortical tubular epithelium of the kidney, and is first detected around 7 days of age. |
Allele Name | transgene insertion M3, Eric G Neilson |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | gammaGT.Cre; gammaGT-Cre; Tg(Ggt1-cre)1Egn |
Gene Symbol and Name | Tg(Ggt1-cre)M3Egn, transgene insertion M3, Eric G Neilson |
Gene Synonym(s) | |
Promoter | Ggt1, gamma-glutamyltransferase 1, rat |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre expression is limited to cortical tubular epithelium of the kidney, and is first detected around 7 days of age. |
Strain of Origin | C57BL/6 x SJL |
Chromosome | UN |
Molecular Note | The rat proximal promoter to Ggt1 was used in a transgene construct to drive the expression of cre recombinase, followed by a termination sequence from human growth hormone. Cre expression was limited to cortical tubular epithelium of the kidney, and was first detected around 7 days of age. |
Mutations Made By | Eric Neilson, Vanderbilt University |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator reports that homozygotes are viable and fertile.
When using the STOCK Tg(Ggt1-cre)M3Egn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012841 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or non carrier for Tg(Ggt1-cre)M3Egn |
Frozen Mouse Embryo | STOCK Tg(Ggt1-cre)M3Egn/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Ggt1-cre)M3Egn/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Ggt1-cre)M3Egn/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Ggt1-cre)M3Egn/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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