Homozygous Fzd3 (frizzled homolog 3 (Drosophila)) targeted mutation mice die at birth due to breathing difficulties which are most likely secondary to central nervous system (CNS) developmental defects. These mice show a complete loss of the thalamocortical, corticothalamic, and nigrostriatal tracts as well as the anterior commissure with variable loss of the corpus callosum. Axonal defects are apparent beginning at embryonic day 13 (E13). Beta galactosidase expression replaces that of the targeted gene. This strain may be useful in studies of neurodevelopment.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Fzd3 | frizzled class receptor 3 |
Homozygous Fzd3 (frizzled homolog 3 (Drosophila)) targeted mutation mice die at birth due to breathing difficulties which are most likely secondary to central nervous system (CNS) developmental defects. These mice show a complete loss of the thalamocortical, corticothalamic, and nigrostriatal tracts as well as the anterior commissure with variable loss of the corpus callosum. Axonal defects are apparent beginning at embryonic day 13 (E13). Peripheral nerve fibers are mostly or completely unaffected. Extensive cell death in the striatum occurs late in gestation, perhaps due to the complete absence of long-range connections. Beta galactosidase expression replaces that of the targeted gene. This strain may be useful in studies of neurodevelopment.
Homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells was used to replace the first two coding exons (exons 1 and 2) of the targeted gene with beta-galactosidase (LacZ) and a neomycin resistance cassette. This strain was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ is expressed in areas of the brain. |
Allele Name | targeted mutation 1, Jeremy Nathans |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | fz3(-) |
Gene Symbol and Name | Fzd3, frizzled class receptor 3 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is expressed in areas of the brain. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 14 |
Molecular Note | The coding regions of the exons 1 and 2 were replaced with a cassette containing lacZ and neo genes. The deleted region encoded the amino terminal signal sequence and a conserved extracellular domain that has been previously been shown to be involved wiht Wnt binding. The expression pattern of beta-galactosidase replicated that of the endogenous gene. |
Mutations Made By | Jeremy Nathans, Johns Hopkins University |
When maintained as a live colony, heterozygotes may be bred. Homozygotes die at birth.
When using the B6;129-Fzd3tm1Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012822 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Fzd3<tm1Nat> |
Frozen Mouse Embryo | B6;129-Fzd3<tm1Nat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Fzd3<tm1Nat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Fzd3<tm1Nat>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Fzd3<tm1Nat>/J Frozen Embryo | $3373.50 |
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