B6;129-Fzd3^tm1Nat/J

Stock number: 012822
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools

Description

Homozygous Fzd3 (frizzled homolog 3 (Drosophila)) targeted mutation mice die at birth due to breathing difficulties which are most likely secondary to central nervous system (CNS) developmental defects. These mice show a complete loss of the thalamocortical, corticothalamic, and nigrostriatal tracts as well as the anterior commissure with variable loss of the corpus callosum. Axonal defects are apparent beginning at embryonic day 13 (E13). Beta galactosidase expression replaces that of the targeted gene. This strain may be useful in studies of neurodevelopment.

Detailed description

Homozygous Fzd3 (frizzled homolog 3 (Drosophila)) targeted mutation mice die at birth due to breathing difficulties which are most likely secondary to central nervous system (CNS) developmental defects. These mice show a complete loss of the thalamocortical, corticothalamic, and nigrostriatal tracts as well as the anterior commissure with variable loss of the corpus callosum. Axonal defects are apparent beginning at embryonic day 13 (E13). Peripheral nerve fibers are mostly or completely unaffected. Extensive cell death in the striatum occurs late in gestation, perhaps due to the complete absence of long-range connections. Beta galactosidase expression replaces that of the targeted gene. This strain may be useful in studies of neurodevelopment.

Development

Homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells was used to replace the first two coding exons (exons 1 and 2) of the targeted gene with beta-galactosidase (LacZ) and a neomycin resistance cassette. This strain was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.

Allele

Symbol: Fzd3^tm1Nat
Name: targeted mutation 1, Jeremy Nathans
MGI accession ID: MGI:2388632
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Fzd3
Marker name: frizzled class receptor 3
Chromosome: 14
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed in areas of the brain.
Molecular note: The coding regions of the exons 1 and 2 were replaced with a cassette containing lacZ and neo genes. The deleted region encoded the amino terminal signal sequence and a conserved extracellular domain that has been previously been shown to be involved wiht Wnt binding. The expression pattern of beta-galactosidase replicated that of the endogenous gene.