C57BL/6-Tg(Thy1-Sncg)HvP36Putt/J

Stock number: 012769
Product name: Thy1mγSN
Research areas: Neurobiology Research

Description

Thy1mγSN transgenic mice have the mouse thymus cell antigen 1 (Thy-1) promoter directing expression of the mouse γ-synuclein (Sncg) gene. These mice may be useful for studying the pathophysiology of Parkinson's disease, and other neurodegenerative disorders.

Detailed description

Thy1mγSN transgenic mice have the mouse thymus cell antigen 1 (Thy-1) promoter directing expression of the mouse γ-synuclein (Sncg) gene. Homozygous Thy1mγSN mice are viable, fertile, and normal in size. They develop a clasping reflex, abnormal posture and gait, and other symptoms of motor dysfunction around 6 months of age, develop limb paralysis by 9 months of age, and die by 16 months of age. Homozygotes also show a 7-fold increase of γ-synuclein mRNA in neurons compared with wild-type mice, leading to motor neuron impairment and premature death. Hemizygous mice develop this phenotype starting at 12 months of age. γ-synuclein aggregates form fibrils, much like α-synuclein, aggregates of which have been associated with neurodegeneration in diseases such as Parkinson's disease. These mice may be useful for studying the pathophysiology of Parkinson's disease, and other neurodegenerative disorders.

Development

The Thy1mγSN transgene was designed to contain the mouse γ-synuclein gene driven by a mouse thymus cell antigen 1 (Thy-1) promoter. The construct was microinjected into fertilized C57BL/6 oocytes. Animals from founder line HvP36 were bred to C57BL/6 mice to establish a colony of hemizygous Thy1mγSN mice. Upon arrival in the laboratory of Dr. Vladimir L. Buchman, these mice were backcrossed to C57BL/6J for 3 generations and were subsequently intercrossed for at least 7 generations to produce homozygotes. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Thy1-Sncg)HvP36Putt
Name: transgene insertion HvP36, Herman van der Putten
MGI accession ID: MGI:4440514
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Thy1-Sncg)HvP36Putt
Marker name: transgene insertion HvP36, Herman van der Putten
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: Sncg,synuclein, gamma,mouse, laboratory
Molecular note: The Thy1mgammaSN transgene was designed to contain the mouse gamma-synuclein gene driven by the neuron-specific mouse thymus cell antigen 1 (Thy-1) promoter. Founder line HvP36 was established.