B6;129S7-Mstn^tm1Swel/J

Stock number: 012685
Research areas: Research Tools

Description

Exon 3 of the Mstn (myostatin) gene is flanked by loxP sites in this targeted mutation strain. Homozygotes for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of muscle growth and maintenance.

Detailed description

Exon 3 of the Mstn (myostatin) gene is flanked by loxP sites in this targeted mutation strain. Homozygotes for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of muscle growth and maintenance.

For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in most tissues (see Stock No. 004682), this mutant mouse strain may be useful in studies of muscle development.

Development

A loxP-flanked neomycin resistance cassette was introduced to intron 3 and a third loxP site was placed in intron 2 of the targeted gene using AB1 129S7/SvEvBrd-Hprt1⁺-derived embryonic stem (ES) cells. The neomycin cassette was excised, leaving the floxed exon 3. This strain was backcrossed minimally to C57BL/6 by the donating laboratory.

Allele

Symbol: Mstn^tm1Swel
Name: targeted mutation 1, Stephen Welle
MGI accession ID: MGI:3711219
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Mstn
Marker name: myostatin
Chromosome: 1
Strain of origin: 129S7/SvEvBrd-Hprt⁺
Molecular note: LoxP sites were inserted to flank exon 3, which encodes the entire active portion of the peptide.