Exon 3 of the Mstn (myostatin) gene is flanked by loxP sites in this targeted mutation strain. Homozygotes for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of muscle growth and maintenance.
Jeffery D. Molkentin, Cincinnati Children's Hospital
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Mstn | myostatin |
Exon 3 of the Mstn (myostatin) gene is flanked by loxP sites in this targeted mutation strain. Homozygotes for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of muscle growth and maintenance.
For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in most tissues (see Stock No. 004682), this mutant mouse strain may be useful in studies of muscle development.
A loxP-flanked neomycin resistance cassette was introduced to intron 3 and a third loxP site was placed in intron 2 of the targeted gene using AB1 129S7/SvEvBrd-Hprt1+-derived embryonic stem (ES) cells. The neomycin cassette was excised, leaving the floxed exon 3. This strain was backcrossed minimally to C57BL/6 by the donating laboratory.
Allele Name | targeted mutation 1, Stephen Welle |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Mstn[f]; Mstnf |
Gene Symbol and Name | Mstn, myostatin |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 1 |
Molecular Note | LoxP sites were inserted to flank exon 3, which encodes the entire active portion of the peptide. |
Mutations Made By | Stephen Welle, University of Rochester Medical Center |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6;129S7-Mstntm1Swel/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012685 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Mstn<tm1Swel> |
Frozen Mouse Embryo | B6;129S7-Mstn<tm1Swel>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Mstn<tm1Swel>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Mstn<tm1Swel>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S7-Mstn<tm1Swel>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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