Leishmania mexicana inoculated PD-L2 (programmed cell death 1, ligand 2) knockout mice exhibit larger lesions, greater parasite burdens than controls as well as elevated levels of IgM and IgG2a. This mutant mouse strain may be useful in studies of immune response.
Arlene H Sharpe, Harvard Medical School
Homozygote: Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Following subcutaneous innoculation with Leishmania mexicana, mutant mice exhibit larger lesions and greater parasite burdens than susceptible 129S4/SvJae controls. Although TH1/TH2 response is similar to control, mutant mice have elevated levels of IgM and IgG2a. This mutant mouse strain may be useful in studies of immune response.
Heterozygote: Not evaluated
A targeting vector containing hygromycin resistance and herpes simplex virus thymidine kinase genes was used to replace the IgV exon of the targeted gene. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to 129S4/SvJae mice and heterozygotes interbred. Upon arrival, mice were bred to 129S4/SvJaeJ for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Arlene H Sharpe|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Pdcd1lg2, programmed cell death 1 ligand 2|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A targeting vector replaced the IgV exon with the hygromycin resistance gene.|
|Mutations Made By|| |
Arlene Sharpe, Harvard Medical School
While maintaining a live colony, these mice are bred as homozygotes.
When using the PD-L2- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32235 in your Materials and Methods section.