Mutant mice on this background will be useful in studies to establish the nature of diabesity modifier genes in the DBA/2 genome as well as the development of diabetic complications, notably diabetic nephropathy and glaucoma.
T1DR Colony, The Jackson Laboratory
Recessive mutations in the leptin receptor produce massive obesity regardless of the strain background on which they are studied. However, development of obesity-induced and insulin resistant diabetes ("diabesity") is strongly influenced by inbred strain background modifier genes and by sex. A comparison of this mutation on multiple inbred backgrounds showed that the congenic transfer of the mutation from the diabesity-susceptible C57BLKS/J background onto the DBA/2J background produced an even more severe diabesity syndrome (Leiter et al., 1981). This is noteworthy because C57BLKS/J is thought to differ from the diabesity-resistant C57BL/6J strain by the presence of DBA/2 genetic contamination in a B6-like genome (Naggert et al., 1995). Unfortunately, this D2.BKS-Leprdb stock was not maintained. Because diabetic DBA/2J males have been shown to be more susceptible to development of diabetic nephropathy (Gurley et al., 2010), speed congenic methodology was used to regenerate this mutant stock. Mutant mice on this background will be useful in studies to establish the nature of diabesity modifier genes in the DBA/2 genome (Davis et al., 2012) as well as the development of diabetic complications, notably diabetic nephropathy and glaucoma.
Heterozygous mice are either grey or dilute in appearance .
The spontaneous autosomal recessive diabetes (Leprdb) mutation was discovered at The Jackson Laboratory, Bar Harbor, Maine USA in 1966 on the inbred strain C57BLKS/J (BKS). Using marker assisted technology, the Type 1 Diabetes Resource and Genetic Resource Sciences established the "D2-db" congenic strain by introducing the Leprdb allele from the BKS.Cg-m +/+ Leprdb/J repulsion stock (see Stock No. 000642) onto the DBA/2J (see Stock No. 000671) background. In 2012, "D2-db" mice generated using speed congenic technology were rederived into the Repository and are at the 6th backcross generation.
|Allele Synonym(s)||Leprdb; diabetes|
|Gene Symbol and Name||Lepr, leptin receptor|
|Gene Synonym(s)||diabetes; OB-R; OB-RGRP; obese-like; obl; OBR; CD295; LEP-R; Leprb; LEPRD; LEPROT; obl; Modb1; leptin receptor gene-related protein; obese-like; db; Fa; Obr|
|Strain of Origin||C57BLKS/J|
|General Note|| |
Phenotypic Similarity to Human Syndrome: Gestational Diabetes J:219658
|Molecular Note||A G-to-T transversion in this allele created a donor splice site that causes abnormal splicing and a 106 nt insertion in the transcript, leading to premature termination of the long cellular domain of the Ob-Rb splice form and loss of its signal transducing function.|
This colony is maintained as segregating heterozygous and wildtype.
When using the D2.BKS(D)-Leprdb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012666 in your Materials and Methods section.
|Heterozygous or wildtype for Lepr<db>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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