Mutant mice on this background will be useful in studies to establish the nature of diabesity modifier genes in the DBA/2 genome as well as the development of diabetic complications, notably diabetic nephropathy and glaucoma.
T1DR Colony, The Jackson Laboratory
Genetic Background | Generation |
---|---|
000671 DBA/2J |
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Lepr | leptin receptor |
Recessive mutations in the leptin receptor produce massive obesity regardless of the strain background on which they are studied. However, development of obesity-induced and insulin resistant diabetes ("diabesity") is strongly influenced by inbred strain background modifier genes and by sex. A comparison of this mutation on multiple inbred backgrounds showed that the congenic transfer of the mutation from the diabesity-susceptible C57BLKS/J background onto the DBA/2J background produced an even more severe diabesity syndrome (Leiter et al., 1981). This is noteworthy because C57BLKS/J is thought to differ from the diabesity-resistant C57BL/6J strain by the presence of DBA/2 genetic contamination in a B6-like genome (Naggert et al., 1995). Unfortunately, this D2.BKS-Leprdb stock was not maintained. Because diabetic DBA/2J males have been shown to be more susceptible to development of diabetic nephropathy (Gurley et al., 2010), speed congenic methodology was used to regenerate this mutant stock. Mutant mice on this background will be useful in studies to establish the nature of diabesity modifier genes in the DBA/2 genome (Davis et al., 2012) as well as the development of diabetic complications, notably diabetic nephropathy and glaucoma.
Heterozygous mice are either grey or dilute in appearance .
The spontaneous autosomal recessive diabetes (Leprdb) mutation was discovered at The Jackson Laboratory, Bar Harbor, Maine USA in 1966 on the inbred strain C57BLKS/J (BKS). Using marker assisted technology, the Type 1 Diabetes Resource and Genetic Resource Sciences established the "D2-db" congenic strain by introducing the Leprdb allele from the BKS.Cg-m +/+ Leprdb/J repulsion stock (see Stock No. 000642) onto the DBA/2J (see Stock No. 000671) background. In 2012, "D2-db" mice generated using speed congenic technology were rederived into the Repository and are at the 6th backcross generation.
Allele Name | diabetes |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | db; db/db; Lepdb; Lepr-; Leprdb-1J; leprdb |
Gene Symbol and Name | Lepr, leptin receptor |
Gene Synonym(s) | |
Strain of Origin | C57BLKS/J |
Chromosome | 4 |
General Note | Phenotypic Similarity to Human Syndrome: Gestational Diabetes J:219658 |
Molecular Note | An intronic G-to-T transversion in this allele created a donor splice site that causes abnormal splicing and the inclusion of 106 nt of intronic sequence in the transcript, leading to premature termination of the long cellular domain of the Ob-Rb splice form and loss of its signal transducing function. |
This colony is maintained as segregating heterozygous and wildtype.
When using the D2.BKS(D)-Leprdb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012666 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Lepr<db> |
Frozen Mouse Embryo | D2.BKS(D)-Lepr<db>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | D2.BKS(D)-Lepr<db>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | D2.BKS(D)-Lepr<db>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | D2.BKS(D)-Lepr<db>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.