This mutation of interferon regulatory factor 6 (Irf6) gene displays abnormal adhesion between the tongue and palate and may be useful studies of cleft palate and Van der Woude Syndrome.
David Beier, Harvard
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Chemically induced (ENU) (Hypomorph) | Irf6 | interferon regulatory factor 6 |
Mice that are homozygous for this hypomorphic allele are characterized by an abnormal adhesion between the tongue and palate. Approximately 63% of E18.5 mutants exhibit a partial fusion of the anterior palate, the remaining mutants exhibit a complete cleft of the secondary palate. Oral adhesions between the palate and tongue are first observed by E12.5. In addition, a small number of mutants exhibit syndactyly, short forelimbs, curly tail, and hind limbs that appear fused to the body.
This mutant mouse strain may be useful in studies of cleft palate and Van der Woude Syndrome.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in A/J males in the laboratory of Dr. David Beier at Brigham and Women's Hospital. Mutagenized males were outcrossed to FVB/NJ females and descendants of the mutagenized males were selected for craniofacial clefting. The mutation results in a change from proline to leucine at amino acid position 39; the same residue mutated in a family with Van der Woude Syndrome. This strain has been backcrossed to FVB/NJ for a minimum 5 of generations. Upon arrival, mice were bred to FVB/NJ for at least 1 generation to establish the colony.
Allele Name | cleft palate 1 |
---|---|
Allele Type | Chemically induced (ENU) (Hypomorph) |
Allele Synonym(s) | |
Gene Symbol and Name | Irf6, interferon regulatory factor 6 |
Gene Synonym(s) | |
Strain of Origin | A/J |
Chromosome | 1 |
Molecular Note | ENU mutagenesis induced a C to T transition that results in the amino acid substitution of lysine for proline at position 39 (P39K). This allele is hypomorphic. |
Mutations Made By | David Beier, Harvard |
While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.
When using the FVB.A-Irf6clft1/BeiJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #012655 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous and wildtype for Irf6<clft1> |
Frozen Mouse Embryo | FVB.A-Irf6<clft1>/BeiJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.A-Irf6<clft1>/BeiJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.A-Irf6<clft1>/BeiJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB.A-Irf6<clft1>/BeiJ Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.