B6.129S7-Pcdhg^tm2Xzw/J

Stock number: 012644
Research areas: Neurobiology Research, Research Tools

Description

These Pcdh-γ^fcon3 mutant mice possess a loxP site upstream of constant exon 3 of gamma-protocadherin (Pcdh-γ) which was fused to EGFP, followed by a loxP-flanked PGK-neomycin resistance (neo) cassette. This strain may be useful for studying neuronal structure and function of hypothalamic neuronal circuitry.

Detailed description

These Pcdh-γ^fcon3 mutant mice possess a loxP site upstream of constant exon 3 of gamma-protocadherin (Pcdh-γ) which was fused to EGFP, followed by a loxP-flanked PGK-neomycin resistance (neo) cassette. Mice that are heterozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have constant exon 3 deleted in cre-expressing tissues, leading to lack of expression of the Pcdh-γ gene cluster. This strain may be useful for studying neuronal structure and function of hypothalamic neuronal circuitry.

For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in study of neurons.

Development

The Pcdh-γ^fcon3 targeting vector was designed to insert a loxP site upstream of constant exon 3 of gamma-protocadherin (Pcdh-γ) which was fused to EGFP, followed by a loxP-flanked PGK-neomycin resistance (neo) cassette. The construct was electroporated into 129S7/SvEvBrd-Hprt1-derived AB2.2 embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were bred to C57BL/6JTyr^c-Brd mice. The donating investigator reported that these mice were backcrossed to C57BL/6JTyr^c-Brd mice (see SNP note below) 5 generations to establish a colony of Pcdh-γ^fcon3 mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Pcdhg^tm2Xzw
Name: targeted mutation 2, Xiaozhong Wang
MGI accession ID: MGI:3821773
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Pcdhg
Marker name: protocadherin gamma cluster
Chromosome: 18
Strain of origin: 129S7/SvEvBrd-Hprt^b-m2
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP is expressed in hypothalamic neurons.
Molecular note: The Pcdh-gamma targeting vector was designed to insert a loxP site upstream of constant exon 3 of gamma-protocadherin (Pcdh-gamma) which was fused to EGFP, followed by a loxP-flanked PGK-neomycin resistance (neo) cassette.