These Pcdh-γfcon3 mutant mice possess a loxP site upstream of constant exon 3 of gamma-protocadherin (Pcdh-γ) which was fused to EGFP, followed by a loxP-flanked PGK-neomycin resistance (neo) cassette. This strain may be useful for studying neuronal structure and function of hypothalamic neuronal circuitry.
Xiaozhong Wang, Northwestern University
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Pcdhg | protocadherin gamma cluster |
These Pcdh-γfcon3 mutant mice possess a loxP site upstream of constant exon 3 of gamma-protocadherin (Pcdh-γ) which was fused to EGFP, followed by a loxP-flanked PGK-neomycin resistance (neo) cassette. Mice that are heterozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have constant exon 3 deleted in cre-expressing tissues, leading to lack of expression of the Pcdh-γ gene cluster. This strain may be useful for studying neuronal structure and function of hypothalamic neuronal circuitry.
For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in study of neurons.
The Pcdh-γfcon3 targeting vector was designed to insert a loxP site upstream of constant exon 3 of gamma-protocadherin (Pcdh-γ) which was fused to EGFP, followed by a loxP-flanked PGK-neomycin resistance (neo) cassette. The construct was electroporated into 129S7/SvEvBrd-Hprt1 A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP is expressed in hypothalamic neurons. |
Allele Name | targeted mutation 2, Xiaozhong Wang |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Pcdhgfcon3; Pcdhgtm2Jrs; Pcdh-gammafcon3 |
Gene Symbol and Name | Pcdhg, protocadherin gamma cluster |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is expressed in hypothalamic neurons. |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 18 |
Molecular Note | The Pcdh-gamma |
Mutations Made By | Xiaozhong Wang, Northwestern University |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or C57BL/6J (Stock No. 000664). The Donating Investigator states that fertility of homozygotes decreases quickly after 3 months of age.
When using the B6.129S7-Pcdhgtm2Xzw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012644 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Wildtype or Heterozygous for Pcdhg<tm2Xzw> |
Frozen Mouse Embryo | B6.129S7-Pcdhg<tm2Xzw>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Pcdhg<tm2Xzw>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Pcdhg<tm2Xzw>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S7-Pcdhg<tm2Xzw>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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