JAX Mouse Strain Datasheet - 012644

B6.129S7-Pcdhg^tm2Xzw/J

Stock No:: 012644

Cryo Recovery

Overview

These Pcdh-γ^fcon3 mutant mice possess a loxP site upstream of constant exon 3 of gamma-protocadherin (Pcdh-γ) which was fused to EGFP, followed by a loxP-flanked PGK-neomycin resistance (neo) cassette. This strain may be useful for studying neuronal structure and function of hypothalamic neuronal circuitry.

Donating Investigator

Xiaozhong Wang, Northwestern University

Genetic overview

Genetic Background	Generation

Pcdhg^tm2Xzw

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Pcdhg	protocadherin gamma cluster

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Research Applications

Neurobiology Research
Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

These Pcdh-γ^fcon3 mutant mice possess a loxP site upstream of constant exon 3 of gamma-protocadherin (Pcdh-γ) which was fused to EGFP, followed by a loxP-flanked PGK-neomycin resistance (neo) cassette. Mice that are heterozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have constant exon 3 deleted in cre-expressing tissues, leading to lack of expression of the Pcdh-γ gene cluster. This strain may be useful for studying neuronal structure and function of hypothalamic neuronal circuitry.

For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in study of neurons.

Development

The Pcdh-γ^fcon3 targeting vector was designed to insert a loxP site upstream of constant exon 3 of gamma-protocadherin (Pcdh-γ) which was fused to EGFP, followed by a loxP-flanked PGK-neomycin resistance (neo) cassette. The construct was electroporated into 129S7/SvEvBrd-Hprt1-derived AB2.2 embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were bred to C57BL/6JTyr^c-Brd mice. The donating investigator reported that these mice were backcrossed to C57BL/6JTyr^c-Brd mice (see SNP note below) 5 generations to establish a colony of Pcdh-γ^fcon3 mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP is expressed in hypothalamic neurons.

Control Suggestions

Wild-type from the colony

Approximate Controls

Additional Information

Considerations for Choosing Controls

Selected References

Lefebvre JL; Zhang Y; Meister M; Wang X; Sanes JR. 2008. {gamma}-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina. Development 135(24):4141-51. PubMed: 19029044 MGI: J:142186

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Genetics

Pcdhg^tm2Xzw

Allele Symbol: Pcdhg^tm2Xzw

Allele Name	targeted mutation 2, Xiaozhong Wang
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Pcdh-gamma^fcon3; Pcdhg^fcon3; Pcdhg^tm2Jrs
Gene Symbol and Name	Pcdhg, protocadherin gamma cluster
Gene Synonym(s)	PCDHG@
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP is expressed in hypothalamic neurons.
Strain of Origin	129S7/SvEvBrd-Hprt
Chromosome	18
Molecular Note	The Pcdh-gamma targeting vector was designed to insert a loxP site upstream of constant exon 3 of gamma-protocadherin (Pcdh-gamma) which was fused to EGFP, followed by a loxP-flanked PGK-neomycin resistance (neo) cassette.
Mutations Made By	Xiaozhong Wang, Northwestern University

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Cre-lox System
  - loxP-flanked Sequences
  - loxP-flanked Sequences: Test/Reporter
- Neurodegeneration
Research Tools
- Genetics Research
  - Tissue/Cell Markers
  - Tissue/Cell Markers: neurons

Genotype: GFP related

Research Tools
- Fluorescent Proteins

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Pcdhg^tm2Xzw/Pcdhg^tm2Xzw
involves: 129S7/SvEvBrd * C57BL/6 * CD-1

nervous system phenotype

abnormal Purkinje cell dendrite morphology
- 6 fold increase in overlaps of Purkinji Cell dendritic branches
- brain injections of rAAV8 virus expressing cre

The following phenotype relates to a compound genotype created using this strain

Genotype: Pcdhg^tm2Xzw/Pcdhg^tm2Xzw Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6J * SJL

vision/eye phenotype

abnormal eye electrophysiology
- retinal ganglion cells fire at a lower rate in response to flicker stimuli and the time course of the light response is lower than in wild-type mice

increased retinal apoptosis
- increased apoptosis is observed in the neuroblast and ganglion cell layers at P0 and is confined to the inner nuclear layer at P7
- retinal apoptosis during the first two weeks after birth is increased compared to in wild-type mice

thin retinal inner nuclear layer
- by P14, 40% thinner than in wild-type mice

thin retinal inner plexiform layer
- by P14, 40% thinner than in wild-type mice

cellular phenotype

increased retinal apoptosis
- increased apoptosis is observed in the neuroblast and ganglion cell layers at P0 and is confined to the inner nuclear layer at P7
- retinal apoptosis during the first two weeks after birth is increased compared to in wild-type mice

References

Lefebvre JL; Zhang Y; Meister M; Wang X; Sanes JR. 2008. {gamma}-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina. Development 135(24):4141-51. PubMed: 19029044 MGI: J:142186

Additional - Pcdhg^tm2Xzw related

Chen WV; Alvarez FJ; Lefebvre JL; Friedman B; Nwakeze C; Geiman E; Smith C; Thu CA; Tapia JC; Tasic B; Sanes JR; Maniatis T. 2012. Functional significance of isoform diversification in the protocadherin gamma gene cluster. Neuron 75(3):402-9. PubMed: 22884324 MGI: J:188341
Garrett AM; Schreiner D; Lobas MA; Weiner JA. 2012. gamma-protocadherins control cortical dendrite arborization by regulating the activity of a FAK/PKC/MARCKS signaling pathway. Neuron 74(2):269-76. PubMed: 22542181 MGI: J:188387
Garrett AM; Weiner JA. 2009. Control of CNS synapse development by {gamma}-protocadherin-mediated astrocyte-neuron contact. J Neurosci 29(38):11723-31. PubMed: 19776259 MGI: J:153046
Gibson DA; Tymanskyj S; Yuan RC; Leung HC; Lefebvre JL; Sanes JR; Chedotal A; Ma L. 2014. Dendrite self-avoidance requires cell-autonomous slit/robo signaling in cerebellar purkinje cells. Neuron 81(5):1040-56. PubMed: 24607227 MGI: J:221288
Kostadinov D; Sanes JR. 2015. Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function. Elife 4:. PubMed: 26140686 MGI: J:225999
Lefebvre JL; Kostadinov D; Chen WV; Maniatis T; Sanes JR. 2012. Protocadherins mediate dendritic self-avoidance in the mammalian nervous system. Nature 488(7412):517-21. PubMed: 22842903 MGI: J:186772
Lobas MA; Helsper L; Vernon CG; Schreiner D; Zhang Y; Holtzman MJ; Thedens DR; Weiner JA. 2012. Molecular heterogeneity in the choroid plexus epithelium: the 22-member gamma-protocadherin family is differentially expressed, apically localized, and implicated in CSF regulation. J Neurochem 120(6):913-27. PubMed: 22092001 MGI: J:183728
Molumby MJ; Keeler AB; Weiner JA. 2016. Homophilic Protocadherin Cell-Cell Interactions Promote Dendrite Complexity. Cell Rep 15(5):1037-50. PubMed: 27117416 MGI: J:235387
Prasad T; Wang X; Gray PA; Weiner JA. 2008. A differential developmental pattern of spinal interneuron apoptosis during synaptogenesis: insights from genetic analyses of the protocadherin-{gamma} gene cluster. Development 135(24):4153-64. PubMed: 19029045 MGI: J:142349
Prasad T; Weiner JA. 2011. Direct and Indirect Regulation of Spinal Cord Ia Afferent Terminal Formation by the gamma-Protocadherins. Front Mol Neurosci 4:54. PubMed: 22275881 MGI: J:190261
Su H; Marcheva B; Meng S; Liang FA; Kohsaka A; Kobayashi Y; Xu AW; Bass J; Wang X. 2010. Gamma-protocadherins regulate the functional integrity of hypothalamic feeding circuitry in mice. Dev Biol 339(1):38-50. PubMed: 20025866 MGI: J:157958

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Wildtype or Heterozygous for Pcdhg<tm2Xzw>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

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General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Pcdhgtm2Xzw

Allele Symbol: Pcdhgtm2Xzw

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: GFP related

Mammalian Phenotype Terms by Genotype

Genotype: Pcdhgtm2Xzw/Pcdhgtm2Xzwinvolves: 129S7/SvEvBrd * C57BL/6 * CD-1

nervous system phenotype

Genotype: Pcdhgtm2Xzw/Pcdhgtm2Xzw Tg(Chx10-EGFP/cre,-ALPP)2Clc/0involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6J * SJL

vision/eye phenotype

cellular phenotype

References

Additional - Pcdhgtm2Xzw related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Pcdhg^tm2Xzw

Allele Symbol: Pcdhg^tm2Xzw

Genotype: Pcdhg^tm2Xzw/Pcdhg^tm2Xzw
involves: 129S7/SvEvBrd * C57BL/6 * CD-1

Genotype: Pcdhg^tm2Xzw/Pcdhg^tm2Xzw Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6J * SJL

Additional - Pcdhg^tm2Xzw related