B6.129-Gria2^tm1Rlh/J

Stock number: 012615
Product name: GluR2 delta 7
Research areas: Neurobiology Research

Description

This mutation of Gria2 (glutamate receptor, ionotropic, AMPA2 (alpha 2); GluR2) incorporates a deletion of the last seven transcribed amino acids, eliminating the C-terminal type II PDZ ligand and disrupting the interaction with PICK1 and GRIP1/2. This strain may be useful for further characterization of the targeted gene's synaptic function.

Detailed description

This mutation of Gria2 (glutamate receptor, ionotropic, AMPA2 (alpha 2); GluR2) incorporates a deletion of the last seven transcribed amino acids, eliminating the C-terminal type II PDZ ligand and disrupting the interaction with PICK1 (protein interacting with C kinase 1) and GRIP1/2 (glutamate receptor interacting proteins 1 and 2). Normal GRIA2 protein expression is replaced by the mutant form in the brain. This strain may be useful for further characterization of the targeted gene's synaptic function.

Development

A loxP-flanked neomycin cassette was inserted into the intron right before the exon encoding the C-terminus region of the targeted gene. An artificial stop codon plus an additional AatII restriction enzyme site was introduced by PCR mutagenesis so as to eliminate the last seven amino acids from the translated protein. The mutation was created using (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Heterozygous mice were bred to CMV-cre transgenic mice to delete the neomycin resistance cassette. The strain was backcrossed to C57BL/6 for 12 generations by the donating laboratory.

Allele

Symbol: Gria2^tm1Rlh
Name: targeted mutation 1, Richard L Huganir
MGI accession ID: MGI:3584143
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Gria2
Marker name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Chromosome: 3
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: An artificial stop codon was inserted upstream of the exon encoding transmembrane 4 and the C terminus region. A floxed neo included in the construct was deleted via cre-mediated recombination. Western blot failed to detect protein using the antibody designed to recognize the last 7 amino acids of the C terminus.