The Stat1- allele has a neo cassette replacing exons 3-5 and part of exon 2 of the signal transducer and activator of transcription 1 gene (Stat1) - abolishing endogenous gene function. These mice may be useful for studying the mechanisms of IFN-induced and STAT1-dependant resistance to infection.
Homozygous (Stat1-/-) mice are viable and fertile when maintained under specific pathogen-free (SPF) conditions. In conventional health status vivaria (non-SPF), homozygotes tend to be runted and die within 48 hours of weaning due to hepatic necrosis due to viral infection.
David E. Levy, New York University
Tiffany A. Reese, Washington University School of Medicine
Genetic Background | Generation |
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N8+pN4F9
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Stat1 | signal transducer and activator of transcription 1 |
The Stat1- allele has a neo cassette replacing exons 3-5 and part of exon 2 of the signal transducer and activator of transcription 1 gene (Stat1) - abolishing endogenous gene function. Homozygous (Stat1-/-) mice are viable and fertile when maintained under specific pathogen-free (SPF) conditions. In conventional health status vivaria (non-SPF), homozygotes tend to be runted and die within 48 hours of weaning due to hepatic necrosis due to viral infection.
Stat1-/- mice fail to mount transcriptional responses to IFNα or IFNγ, as shown by lack of induction of normal IFN inducible genes ISG54, IRF-1, or ISGF3γp48. STAT1 response results in abnormal innate immunity, but may be redundant in pathways other than IFN due to various compensatory mechanisms. These mice may be useful for studying the mechanisms of INF-induced and STAT1-dependant resistance to infection.
A targeting vector was designed by Dr. David E. Levy (New York University School of Medicine) to replace exons 3-5 and part of exon 2 encoding the signal transducer and activator of transcription 1 (Stat1) gene with a neomycin resistance (neo) cassette in reverse orientation to the gene. The construct was electroporated into 129S/SvEv-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females and subsequently to CD1 mice to generate a colony of Stat1-/- mice. These mice were backcrossed 8 generations to C57BL/6 background and following micro satellite marker analysis 100% C57BL/6 animals were selected for subsequent interbreeding. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, David E Levy |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | STAT-; STAT1-; Stat1 KO |
Gene Symbol and Name | Stat1, signal transducer and activator of transcription 1 |
Gene Synonym(s) | |
Strain of Origin | 129S/SvEv-Gpi1c |
Chromosome | 1 |
Molecular Note | A neomycin resistance cassette replaced 5.7 kb of sequence, including 3 exons and a portion of a fourth encoding amino acids 221-365. This allele produces a partial nonfunctional protein product. |
Mutations Made By | David Levy, New York University |
Homozygous (Stat1-/-) mice are viable and fertile when maintained under specific pathogen-free (SPF) conditions. In conventional health status vivaria (non-SPF), homozygotes tend to be runted and die within 48 hours of weaning due to hepatic necrosis due to viral infection.
Because The Jackson Laboratory Stock No. 012606 live colony is maintained in a high-SPF facility, it is our experience that both heterozygous and homozygous mice appear healthy and well. As such, we maintain our live colony by breeding heterozygous mice together, to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). We may also attempt to breed heterozygotes with homozygotes (September 2018).
When using the Stat1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #012606 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Stat1<tm1Dlv |
Frozen Mouse Embryo | B6.129S(Cg)-Stat1<tm1Dlv>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S(Cg)-Stat1<tm1Dlv>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S(Cg)-Stat1<tm1Dlv>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S(Cg)-Stat1<tm1Dlv>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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