B6;129-Tgfbr2^tm1Karl/J

Stock number: 012603
Product name: TβRII
Research areas: Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 4 of the transforming growth factor, beta receptor II (Tgfbr2) (commonly referred to as TβRII) targeted gene. This strain may be useful for studying the cellular and mechanical role of TGF-β in regulating development, hematopoiesis, wound healing, and immune function.

Detailed description

These TβRII floxed mutant mice possess loxP sites flanking exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in cre-expressing tissues. This strain may be useful for studying the cellular and mechanical role of TGF-β in regulating development, hematopoiesis, wound healing, and immune function.

For example, when crossed to a strain expressing Cre recombinase in the neural tube, midbrain and dorsal spinal cord (see Stock No. 007807), this mutant mouse strain may be useful in studies of DiGeorge syndrome.

For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of autoimmune inflammation.

 

Development

A targeting vector was designed to insert a loxP-flanked neomycin resistance (neo) cassette upstream of exon 4, and a single loxP site downstream of exon 4 of the transforming growth factor beta (TGF-β) type II receptor (Tgfbr2) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pIC-Cre expression plasmid to delete the neo cassette. Resulting ES cells contained multiple gene rearrangments; intact floxed-exon 4, intact floxed-neo cassette, or excision of both exon 4 and the neo cassette. Correctly targeted ES cells, containing only the floxed-exon 4, were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6 females. Resulting offspring were bred to establish a colony of TβRII mice. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tgfbr2^tm1Karl
Name: targeted mutation 1, Stefan Karlsson
MGI accession ID: MGI:2183502
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Tgfbr2
Marker name: transforming growth factor, beta receptor II
Chromosome: 9
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exon 4 was left flanked by single loxP sites in introns 3 and 5 after a floxed neo cassette was excised via cre-mediated recombination in ES cells.