B6.Cg-Tg(TH-ALPP)1Erav/J

Stock number: 012588
Research areas: Neurobiology Research, Sensorineural Research

Description

THpPLAP transgenic mice have the rat tyrosine hydroxylase (TH) promoter directing expression of the human Placental Alkaline Phosphatase (ALPP or hPLAP) gene. These mice may be useful for visualizing DA cells, and correlating their physiological properties with their synaptic connections by use of microscopy, electrophysiology, and molecular biology, and for studying the pathophysiology of Parkinson's disease, and other neurodegenerative disorders.

Detailed description

THpPLAP transgenic mice have the rat tyrosine hydroxylase (TH) promoter directing expression of the human Placental Alkaline Phosphatase (ALPP or hPLAP) gene. Homozygous THpPLAP mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. PLAP intensely labels the outer surface of the cell membrane of TH-expressing dopamine (DA) releasing, catecholaminergic amacrines of the retina and the dopaminergic neurons of the brain, including those of the substantia nigra, VTA and olfactory bulb. Furthermore they label, less intensely, the neurons of the locus coeruleus (norepinephrine) and, weakly, the type 2 catecholaminergic amacrines of the retina (transmitter unknown). These mice may be useful for visualizing DA cells, and correlating their physiological properties with their synaptic connections by use of microscopy, electrophysiology, and molecular biology, and for studying the pathophysiology of Parkinson's disease, and other neurodegenerative disorders.

Development

The THpPLAP transgene was designed with the human Placental Alkaline Phosphatase (ALPP or hPLAP) gene driven by a rat tyrosine hydroxylase (TH) promoter, a simian virus 40 (SV40) intervening sequence, and SV40 polyadenylation site. The transgene was microinjected into fertilized C57BL/6 X SJL/J F2 oocytes, and founder mice were bred to C57BL/6J mice (see SNP note below) for at least eight generations to establish a colony prior to arrival. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Allele

Symbol: Tg(Th-ALPP)1Erav
Name: transgene insertion 1, Elio Raviola
MGI accession ID: MGI:4436729
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Th-ALPP)1Erav
Marker name: transgene insertion 1, Elio Raviola
Chromosome: UN
Strain of origin: (C57BL/6 x SJL/J)F2
Expressed genes: ALPP,alkaline phosphatase, placental,human
Molecular note: The THpPLAP transgene was designed with the human Placental Alkaline Phosphatase (ALPP or hPLAP) gene driven by a rat tyrosine hydroxylase (TH) promoter, a simian virus 40 (SV40) intervening sequence, and SV40 polyadenylation site. This line contains 50 copies of the transgene. PLAP intensely labels the outer surface of the cell membrane of TH-expressing dopamine (DA) releasing, catecholaminergic amacrines of the retina and the dopaminergic neurons of the brain, including those of the substantia nigra, VTA and olfactory bulb. Furthermore they label, less intensely, the neurons of the locus coeruleus (norepinephrine) and, weakly, the type 2 catecholaminergic amacrines of the retina (transmitter unknown).