Mice homozygous for this Dgcr82lox (or Dgcr8flox) conditional allele are viable and fertile, with loxP sites flanking exon 3 of the Dgcr8 (DiGeorge syndrome critical region gene 8) locus. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in cre-expressing tissues. Such a deletion of exon 3 generates a frameshift mutation with multiple downstream premature stop codons resulting in a truncated protein missing the WW protein-protein interaction and RNA-binding domains. This deletion will disrupt Drosha/Dgcr8-dependent cleavage of precursor miRNAs in the nucleus and thus inhibit canonical microRNA biogenesis.

These floxed mutant mice harbor loxP sites flanking exon 3 of the DiGeorge syndrome critical region gene 8 (Dgcr8) gene and may be useful in generating conditional mutations for studying the role of Dgcr8 in microRNA regulation.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.