Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from cryorecovery.
These floxed mutant mice harbor loxP sites flanking exon 3 of the DiGeorge syndrome critical region gene 8 (Dgcr8) gene and may be useful in generating conditional mutations for studying the role of Dgcr8 in microRNA regulation.Robert Blelloch, University of California, San Francisco
Genetic Background | Generation |
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N?+N2F6
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Dgcr8 | DGCR8, microprocessor complex subunit |
Mice homozygous for this Dgcr82lox (or Dgcr8flox) conditional allele are viable and fertile, with loxP sites flanking exon 3 of the Dgcr8 (DiGeorge syndrome critical region gene 8) locus. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in cre-expressing tissues. Such a deletion of exon 3 generates a frameshift mutation with multiple downstream premature stop codons resulting in a truncated protein missing the WW protein-protein interaction and RNA-binding domains. This deletion will disrupt Drosha/Dgcr8-dependent cleavage of precursor miRNAs in the nucleus and thus inhibit canonical microRNA biogenesis.
A targeting construct was designed to insert a loxP site upstream of exon 3, and a loxP-flanked CMV-HygroTK selection cassette downstream of exon 3 of the Dgcr8 (DiGeorge syndrome critical region gene 8) locus. This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a Cre recombinase-expressing plasmid. The resulting ES cells with the Dgcr82lox (Dgcr8flox) genotype (CMV-HygroTK selection cassette removed; leaving a single loxP site upstream of exon 3 and a single loxP site downstream of exon 3) were injected into recipient blastocysts. Chimeric mice were bred with C57BL/6NCr inbred mice to generate the Dgcr82lox colony. Mutant mice were backcrossed to C57BL/6NCr for eight generations, and thereafter maintained by breeding homozygous mice together for many generations prior to sending to the MMRRC at The Jackson Laboratory Repository. Upon arrival, mice were bred with C57BL/6NJ inbred mice for at least one generation to establish the colony.
Allele Name | targeted mutation 1.1, Robert Blelloch |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Dgcr2lox |
Gene Symbol and Name | Dgcr8, DGCR8, microprocessor complex subunit |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 16 |
Molecular Note | A loxP site was inserted in intron 2 and a floxed CMV-Hygro selection cassette was inserted in intron 3. ES cells were sequentially targeted so that ES cells carried two mutated alleles. Cre recombinase treatment of these ES cells resulted in two mutant alleles, this one with a floxed exon 3 but lacking the selection cassette and another allele which lacks both exon 3 and the selection cassette. |
Mutations Made By | Robert Blelloch, University of California, San Francisco |
When maintaining a live colony, homozygous mice may be bred together.
When using the Dgcr2lox mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32051 in your Materials and Methods section.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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