These hemizygous transgenic mice carry a human synuclein (alpha-syn) gene, SNCA, regulated by a tetracycline operator (tetO). When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of SNCA protein may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. These mice may be useful for studying the Parkinson's disease pathogenesis and neurodegeneration elicited by the toxic effects of aggregation and somatic accumulation of SNCA.
Huaibin Cai, National Institutes of Health / National Institute on Aging (NIH/NIA)
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
Mice hemizygous for the human synuclein (alpha-syn) transgene, (SNCA) are viable and fertile. Expression of the transgene is regulated by the tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator). When mated to a strain expressing tetracycline-controlled transactivator protein (tTA), expression of the SNCA protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. The formation of alpha-syn aggregates is a key step in the pathogenesis of Parkinson's disease. These aggregates cause formation of fibrillar aggregates causing various forms of cytotoxicity, including impairment of proteasomal and lysosomal activities, disruption of ER-Golgi traffic and microtubule-based transport, and dysfunction of mitochondria. SNCA mice develop age-dependent, progressive neurodegeneration. These mice may be useful for studying the Parkinson's disease pathogenesis and neurodegeneration elicited by the toxic effects of aggregation and somatic accumulation of SNCA.
For example, when bred to a strain expressing rtTA or tTA in forebrain neurons (see Stock No. 007004 for example), this mutant mouse strain may be useful in studies of neurodegeneration.
A transgenic vector was generated encoding the C-terminal hemagglutinin (HA)-tagged human SNCA gene under control of a tetO promoter. The construct was microinjected into C57BL/6J fertilized oocytes, and founder mice were bred to C57BL/6J mice (see SNP note below) to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 6 markers were found to be segregating for DBA/J from an unknown source.
Expressed Gene | SNCA, synuclein alpha, human |
---|---|
Site of Expression |
Allele Name | transgene insertion 1, Huaibin Cai |
---|---|
Allele Type | Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | alpha-syn WT |
Gene Symbol and Name | Tg(tetO-SNCA)1Cai, transgene insertion 1, Huaibin Cai |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | SNCA, synuclein alpha, human |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | A transgenic vector was generated encoding the human SNCA gene under control of a tetO promoter. The construct was microinjected into fertilized oocytes, and a line of mice was established. |
Mutations Made By | Huaibin Cai, National Institutes of Health / National Institute on Aging (NIH/NIA) |
When maintained as a live colony, homozygous mice may be bred together or transgene carrier mice may be bred to wild type siblings.
When using the B6;D2-Tg(tetO-SNCA)1Cai/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012450 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(tetO-SNCA)1Cai |
Frozen Mouse Embryo | B6;D2-Tg(tetO-SNCA)1Cai/J | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(tetO-SNCA)1Cai/J | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(tetO-SNCA)1Cai/J | $3373.50 |
Frozen Mouse Embryo | B6;D2-Tg(tetO-SNCA)1Cai/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.