These hemizygous transgenic mice carry a human leucine-rich repeat kinase 2 gene, LRRK2, regulated by a tetracycline operator (tetO). When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of LRRK2 protein may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. These mice may be useful for studying the Parkinson's disease pathogenesis and neurodegeneration.
Huaibin Cai, National Institutes of Health / National Institute on Aging (NIH/NIA)
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
Mice hemizygous for the human leucine-rich repeat kinase 2 transgene, (LRRK2), are viable and fertile. Expression of LRRK2 is regulated by a tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator). When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of LRRK2 protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. LRRK2 protein, also known as Dardarin, contains multiple functional domains and may function as both an active GTPase and kinase. LRRK2 may regulate alpha-synuclein-mediated neuropathology through modulating the intracellular trafficking and accumulation of SNCA protein. These mice may be useful for studying Parkinson's disease pathogenesis and neurodegeneration.
A transgenic vector was generated encoding the C-terminal hemagglutinin (HA)-tagged human LRRK2 gene under control of a tetO promoter. The construct was microinjected into C57BL/6J fertilized oocytes, and founder mice were bred to C57BL/6J mice to establish a colony (see below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed 6 of 32 markers (on six different chromosomes) that were not C57BL/6J allele-type. These data suggest C57BL/6N, FVB, and/or other genetic contamination prior to arrival at The Jackson Laboratory.
Expressed Gene | LRRK2, leucine rich repeat kinase 2, human |
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Site of Expression |
Allele Name | transgene insertion C7874, Huaibin Cai |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | tetO-LRRK2 |
Gene Symbol and Name | Tg(tetO-LRRK2)C7874Cai, transgene insertion C7874, Huaibin Cai |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | LRRK2, leucine rich repeat kinase 2, human |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | A transgenic vector was generated encoding the C-terminal hemagglutinin (HA)-tagged human LRRK2 gene under control of a tetO promoter. The construct was microinjected into C57BL/6J fertilized oocytes, and 5 independent lines were generated including line C7874. Founder mice were bred to C57BL/6J mice to establish this colony. |
Mutations Made By | Huaibin Cai, National Institutes of Health / National Institute on Aging (NIH/NIA) |
When maintained as a live colony homozygous mice may be bred together.
When using the STOCK Tg(teto-LRRK2)C7874Cai/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012449 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(tetO-LRRK2)C7874Cai |
Frozen Mouse Embryo | STOCK Tg(teto-LRRK2)C7874Cai/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(teto-LRRK2)C7874Cai/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(teto-LRRK2)C7874Cai/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(teto-LRRK2)C7874Cai/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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