These transgenic mice carry a humanized synuclein (alpha-syn) mutant (SNCA*A53T) gene regulated by a tetracycline operator (tetO). When mated to a mutant strain expressing tTA, expression of SNCA*A53T protein may be regulated with the tetracycline analog doxycycline in the double mutant offspring.
Huaibin Cai, National Institutes of Health / National Institute on Aging (NIH/NIA)
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
Mice hemizygous for the human synuclein (alpha-syn) mutant transgene, (SNCA*A53T) are viable and fertile. Expression of SNCA*A53T is regulated by the tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator). When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of SNCA*A53T protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. Mutations in alpha-syn greatly accelerate the formation of fibrillar aggregates causing various forms of cytotoxicity, including impairment of proteasomal and lysosomal activities, disruption of ER-Golgi traffic and microtubule-based transport, and dysfunction of mitochondria. SNCA*A53T mice develop age-dependent, progressive neurodegeneration. These mice may be useful for studying the Parkinson's disease pathogenesis and neurodegeneration elicited by the toxic effects of aggregation and somatic accumulation of SNCA.
A transgene was generated encoding a human SNCA*A53T mutant gene under control of a tetO promoter. The construct was microinjected into C57BL/6J fertilized oocytes, and founder mice were bred to C57BL/6J mice to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed 10 of 32 markers (on six different chromosomes) that were not C57BL/6J allele-type. These data suggest C57BL/6N, FVB, and/or other genetic contamination prior to arrival at The Jackson Laboratory.
Expressed Gene | SNCA, synuclein alpha, human |
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Site of Expression |
Allele Name | transgene insertion E2, Huaibin Cai |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | alpha-syn A53T; tetO-A53T |
Gene Symbol and Name | Tg(tetO-SNCA*A53T)E2Cai, transgene insertion E2, Huaibin Cai |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | SNCA, synuclein alpha, human |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | A transgenic vector was generated encoding a human SNCA*A53T mutant gene under control of a tetO promoter. The construct was microinjected into C57BL/6J fertilized oocytes, and founder mice were bred to C57BL/6J mice to establish the E2 line. |
Mutations Made By | Huaibin Cai, National Institutes of Health / National Institute on Aging (NIH/NIA) |
When maintained as a live colony, homozygous mice may be bred together or transgene carrier mice may be bred to wild type siblings.
When using the STOCK Tg(tetO-SNCA*A53T)E2Cai/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012442 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(tetO-SNCA*A53T)E2Cai |
Frozen Mouse Embryo | STOCK Tg(tetO-SNCA*A53T)E2Cai/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(tetO-SNCA*A53T)E2Cai/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(tetO-SNCA*A53T)E2Cai/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(tetO-SNCA*A53T)E2Cai/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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